Cell & Gene Therapy Resources

The authors used a Moxi GO II to count and monitor CAR T cells (including cell size/growth kinetics) throughout expansion, advancing the products into in vitro cytotoxicity assays and in vivo PTCL PDX studies only once the cells’ kinetics/size indicated they had “rested” from stimulation.

The authors used a Moxi GO II to count engineered T-cell cultures every other day and monitor cell size/growth kinetics as a practical criterion for when the cells had rested from stimulation and were ready to proceed into downstream adoptive T-cell experiments.

Scientists are concerned with speed, accuracy, and convenience, and those running the lab and industries are concerned with the cost typically associated with high-performing instruments. Our proprietary Coulter Principle-based system delivers on all three accounts, and is therefore a perfect fit for any cell biology benchtop.

There are a variety of ways to monitor this process in cell culture, the two most prominent being cell counting via imaging-based and coulter-based cell counting methods and higher-level cellular analysis with fluorophore-tagged antibodies and fluorescent reporters via flow cytometry. While each instrument used to achieve these two things has its advantages and disadvantages, few and far between can do both at once. Out of all your options, no other instrument has the power to do both with the speed, precision, and ease that Precision Cell Systems' Moxi Go II can. In this document, we will take you through how you can unlock the next stage in your cellular analysis process for CAR-T with our instruments.