Application Notes

Much of the hassle of analyzing PBMC samples comes from the inadequate counting of the cells of interest, usually because of debris or lack of robust counting. By circumventing imaging and going back to gold-standard physics-based principles, every Moxi instrument gives unparalleled abilities in getting the best cell count. With the added opportunities for fluorescence-based detection with a Moxi V and Moxi GO II, PBMC viability checks have never been more accurate. Ensure your precious samples aren’t wasted by making sure you get the quality checks right the first time by relying on a Moxi instrument.

Scientists are concerned with speed, accuracy, and convenience, and those running the lab and industries are concerned with the cost typically associated with high-performing instruments. Our proprietary Coulter Principle-based system delivers on all three accounts, and is therefore a perfect fit for any cell biology benchtop.

There are a variety of ways to monitor this process in cell culture, the two most prominent being cell counting via imaging-based and coulter-based cell counting methods and higher-level cellular analysis with fluorophore-tagged antibodies and fluorescent reporters via flow cytometry. While each instrument used to achieve these two things has its advantages and disadvantages, few and far between can do both at once. Out of all your options, no other instrument has the power to do both with the speed, precision, and ease that Precision Cell Systems' Moxi Go II can. In this document, we will take you through how you can unlock the next stage in your cellular analysis process for CAR-T with our instruments.

Protocols for single-cell sequencing library preparation present several challenges along the way that can impact the accuracy and quality of downstream sequencing results. Making sure you overcome these is essential for not wasting valuable sample and money on failed sequencing runs. Precision Cell Systems’ Moxi line of Coulter Principle-based cell analyzers enable you to address all of these variables in a single instrument, making it the ideal go-to for all of your sequencing needs.

Here, we present the Precision Cell Systems Moxi GO II system as a simple, rapid, and effective flow cytometric approach to the study of apoptosis using Annexin V

The triple-layered cell health assessment where the first layer of isolating cells from debris via event size, the second layer of PI for staining dead cells, and the third layer of AO for all cells or calcein for only live cells ensures that every event that is a real cell has been classified as alive or dead and none are left uncounted. The less layers that are used, the more unreliable the results. Multiple cell counters on the market are capable of doing this, but the accuracy and repeatability of data generated by a Moxi Go II makes it the clear winner.

The Singulator platform paired with Parse Biosciences technology enables efficient single-cell isolation and sequencing from challenging tissue types, with high viability and robust cell yields suitable for downstream scRNA-seq analysis.

These data demonstrate the ability of the Singulator Platform to prepare single-cell suspensions for both positive selection by a cell surface marker and single-cell RNA sequencing. These methods enable researchers to identify genes of interest for potential therapeutic targets for anti-tumor response

The Singulator™ Platform provides a reproducible and precise method for isolating nuclei from complex tissues suitable for single-nuclei RNA sequencing. Our results demonstrate consistent nuclei yield and high-quality sequencing metrics across biological replicates, making it a reliable tool for neuroscientific research. The ability to produce high-quality nuclei with minimal variability enhances the accuracy of downstream snRNA analyses, facilitating deeper insights into normal biology and disease. Finally, the ability to identify rare cell types underscores the efficacy of the Singulator Platform in comprehensive cellular analysis, providing a detailed representation of the cellular landscape.

The presence of intracellular and extracellular debris in single-nucleus RNA sequencing can present a significant challenge in obtaining reliable and accurate results. However, the use of the Precision Cell Systems Nuclei Debris Removal Reagent can effectively improve the quality of the samples. Our evaluation of the Nuclei Debris Removal Reagent shows successful removal of debris from traditionally high-debris samples of brain and liver nuclei while delivering high quality single nuclei suspensions. The resulting samples are able to be run through microfluidic single nuclei sequencing platforms without issues of clogging or reduced data quality