PublicationPublications & Research
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PublicationCatalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells
In this study, the authors used a Moxi Z Cell Counter to count trypsinized, treated PANC-1 cells so they could re-plate defined cell numbers for colony formation assays and accurately calculate plating efficiency and survival fraction after PAA-TiOx nanoparticle and X-ray treatments under normoxic and hypoxic conditions.
PublicationSynthesis and Application of Nanovanilloids for Localized Therapeutic Hypothermia after Traumatic Brain Injury
The authors used a Moxi Z to count cells as part of preparing mammalian cell cultures for in vitro nanovanilloid testing (viability/oxidative-stress assays and TRPV1 calcium-influx imaging).
PublicationARNT-dependent HIF-2α signaling protects cardiac microvascular barrier integrity and heart function post-myocardial infarction
In this study, the authors used the Moxi GO II to quantify endothelial apoptosis after Hif2α siRNA knockdown and hypoxic exposure, using Annexin V–FITC and PI with the instrument’s apoptosis app to measure cell-death outcomes under their ischemia-relevant conditions.
PublicationDevice-free isolation of photoreceptor cells from patient iPSC-derived retinal organoids
In your staged partial-dissociation retinal organoid workflow, you used the Moxi Go II to count the filtered dissociation-fraction cell suspensions immediately before 10x Chromium X scRNA-seq loading (targeting ~8,000 cells per sample), enabling consistent single-cell capture across fractions and lines.
PublicationBinary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms
The authors used a Moxi V to measure Aspergillus niger spore concentration, enabling per-10^6-spore normalization of downstream plate-reader GFP fluorescence when comparing transformants across binary vector ORI variants.
PublicationInduction of a transcriptional adaptation response by RNA destabilization events
The authors used the MOXI Z Mini to count and standardize 50,000 NIH3T3 cells per condition immediately before ATAC-seq library preparation, supporting their chromatin-accessibility comparisons at the Actg2 locus across RNA-destabilizing perturbations.
PublicationTranscriptome profiling of peripheral blood mononuclear cells from highly susceptible adult cattle infected with a virulent strain of Babesia bovis
The authors used a Moxi Z to count isolated bovine PBMCs after density separation and washes, then normalized each sample to ~2×10^7 cells per tube prior to RNAlater stabilization and downstream bulk RNA-Seq.
PublicationMitochondrial Transfer from Human Platelets to Rat Dental Pulp-Derived Fibroblasts in the 2D In Vitro System: Additional Implication in PRP Therapy
The author used a platelet–fibroblast co-culture model, and a Moxi-Z Coulter-based cell counter to quantify RPC-C2A fibroblast cell numbers after UVC injury and platelet/lysate treatment and to normalize fibroblast-associated ATP measurements to cell count, supporting your survival and bioenergetic conclusions.
PublicationSecretion of specific metabolites and changes in miRNA expression in murine osteoblastic cells exposed in vitro to α-radiation
The authors used a Moxi GO II to perform fluorescence-based flow cytometric phenotyping of BM-derived cultures (CD45 and intracellular RUNX2/BAP) to verify the osteoblastic differentiation context for interpreting downstream α-radiation–responsive omics measurements.
Publication5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer
In this study, the authors used the Moxi GO II to run a FITC-based apoptosis assay on treated prostate cancer cells (±5-ALA, ±irradiation) and quantify early apoptotic fractions as a mechanistic readout of radiosensitization.
PublicationThe transcription of a single olfactory receptor per neuron is enforced by epigenetic silencing of their enhancers
The authors used a Moxi cell counter (Orflo, S cassettes) to quantify the live, post-FACS-sorted MOE single-cell suspension and dilute it to ~1000 cells/µL for 10x Genomics single-cell RNA-seq loading.
PublicationProliferation of a bloom-forming phytoplankton via uptake of polyphosphate-accumulating bacteria under phosphate-limiting conditions
In this study, the authors used a Moxi Z to quantify H. akashiwo cell-density changes in phosphate-depleted co-cultures and a Moxi Go II Mini to measure CellTracker™ Green–labeled bacterial fluorescence associated with algal-sized, red-autofluorescent particles as evidence of bacterivory linked to growth rescue under phosphate limitation.
PublicationTubulin sequence divergence is associated with the use of distinct microtubule regulators
In this study of Naegleria’s mitotic vs differentiation microtubule programs, the authors used a Moxi Z impedance-based counter to quantify amoeba concentration so they could inoculate and grow cultures at defined densities before initiating the mitotic synchronization workflow.
PublicationSmall-scale protocols to characterize mitochondrial Complex V activity and assembly in peripheral blood mononuclear cells
In this study, the authors used a Moxi GO II (561 nm LP filter) to quantify TMRM fluorescence in PBMCs and calculate inhibitor-dependent shifts in the fraction of TMRM-positive cells as a small-sample, intact-cell readout of mitochondrial membrane potential (∆Ѱm) and Complex V operating mode.
PublicationLaser-driven VHEE pulsed fast fractionation (PFF): second-scale inter-pulse timing differentially modulates normal tissue and tumour toxicity
The authors used an ORFLO Moxi Mini to count viable cells after irradiated MRC5-hTERT and HCT116 pellets were re-plated and expanded for 5–6 days, using the averaged viable-cell counts (normalized to same-day non-irradiated controls) as the viability endpoint to compare inter-pulse timing conditions.
PublicationSelective Inhibition of Vascular Smooth Muscle Cell Function by COVID-19 Antiviral Drugs: Impact of Heme Oxygenase-1
The authors used a Moxi Z to count trypsinized vascular smooth muscle cells after a 3-day antiviral treatment, providing the primary quantitative endpoint for proliferation in their remdesivir vs. molnupiravir/nirmatrelvir comparisons.
PublicationBmpr2 Loss in the Vascular Endothelium Enhances Neoangiogenesis and Growth of Lung Metastatic Lesions in a Mouse Model of Breast Cancer
The authors used a Moxi Z (Type-M cassettes) to collect 24-hour interval EO771 cell counts and build proliferation curves to test whether BMP9 treatment or BMPR2-silenced endothelial conditioned media/ECM altered EO771 growth.
PublicationNKX2. 2 and KLF4 cooperate to regulate α-cell identity
In this study, the authors used a Moxi GO II with propidium iodide to measure cell count and viability of αTC cells after Klf4 siRNA transfection, supporting the subsequent qPCR-based knockdown validation and selection of knockdown samples for downstream mechanistic assays.
PublicationTumor-treating fields and concurrent cisplatin: an in vitro demonstration of efficacy in triple-negative breast cancer
In this TNBC cisplatin in vitro study, the authors used the Moxi GO II to run an Annexin V (FITC) / PI apoptosis flow-cytometry assay (and to capture 24-hour flow-based cell-count outcomes) to quantitatively compare treatment-induced killing in TNBC versus epithelial cells across mono-culture and co-culture conditions.
PublicationA patient-derived T cell lymphoma biorepository uncovers pathogenetic mechanisms and host-related therapeutic vulnerabilities
The authors used a Moxi GO II to count and monitor CAR T cells (including cell size/growth kinetics) throughout expansion, advancing the products into in vitro cytotoxicity assays and in vivo PTCL PDX studies only once the cells’ kinetics/size indicated they had “rested” from stimulation.
PublicationInvestigating Nanoparticle Distribution and Tissue Architecture in Male and Female Human Cardiac Organoids
The authors used a Moxi cell counter to accurately seed 10,000 iPSCs per well when generating human cardiac organoids, ensuring consistent starting organoid inputs before assessing fluorescent nanoparticle biodistribution and cell-type–specific uptake by downstream fluorescence microscopy.
PublicationPulmonary Vascular Endothelial Dysfunction Is Induced by Nonpulsatile Pulmonary Blood Flow in an Ovine Classic Glenn Model
The authors used a Moxi Z to count primary pulmonary artery endothelial cells after trypsinization—both to seed 6000 cells/well and to perform serial 24-hour cell counts through 120 hours—to quantify and compare PAEC proliferation between control and Glenn lambs.
PublicationCharacterization of the Intact Proteomic Profile of Senescent-Associated Secretory Phenotype by Top-Down Mass Spectrometry
The authors detached IMR-90 fibroblasts with trypsin and used a Moxi V cell analyzer to count the cells, with no further stated use of the Moxi readout for secretome normalization in the proteomics workflow.
PublicationMaternal acute SARS-CoV-2 infection impairs preimplantation embryo development and reprograms the early offspring hematopoietic system
The authors used the Moxi GO II to measure cell concentration and ensure optimal loading density when preparing neonatal umbilical cord blood single-cell suspensions for 10× Genomics Chromium scRNA-seq.cytometer (Orflo Technologies) to ensure optimal loading density.
PublicationEZH1/EZH2 inhibition enhances adoptive T cell immunotherapy against multiple cancer models
The authors used a Moxi GO II to count engineered T-cell cultures every other day and monitor cell size/growth kinetics as a practical criterion for when the cells had rested from stimulation and were ready to proceed into downstream adoptive T-cell experiments.
PublicationAttenuation of HIV-specific T cell responses Among people with HIV on ART following dipyridamole treatment
The authors used a Moxi Go II to verify post-thaw PBMC viability and accurately count cells so they could plate a consistent 1×10⁶ cells per condition for HIV Gag/Env peptide stimulation prior to intracellular cytokine staining and polyfunctionality analysis by flow cytometry.
PublicationNectin-4 Tumor Expression as a Prognostic Factor and Potential Therapeutic Target in Stage II/III Rectal Cancer
The authors used the Moxi GO II to measure PE-labeled cell-surface Nectin-4 expression by flow cytometry in a rectal cancer cell line, providing protein-level confirmation of Nectin-4 knockdown used to interpret downstream proliferation/5-FU response assays.
PublicationEarly-life microRNA signatures in cord blood associated with allergic rhinitis and asthma development
In this study, the authors used a Moxi Z to count human airway smooth muscle cells 72 hours after miRNA mimic transfection, enabling them to quantify relative proliferation vs scramble control as functional validation for asthma- and rhinitis-associated cord-blood miRNA signatures.
PublicationMajor membrane protein of Mycobacterium avium subp. paratuberculosis activates immune and autophagic pathways in bovine monocyte-derived macrophages
The authors used a Moxi Z to count CD14+ bovine monocytes after magnetic enrichment and standardize seeding concentration prior to ex vivo differentiation and downstream antigen-stimulation readouts (MAPK ELISA and RNA-seq).
PublicationComparative Analysis of Inflammatory Response in Surgical Wound Drainage Fluid in Scoliosis Surgery: A Study of Neuromuscular vs. Idiopathic Patients
In this scoliosis wound-healing study, the authors used a Moxi Z to count cells recovered from POD1 surgical wound drainage after RBC lysis, then used that counted cell suspension as the input for multicolor flow-cytometry immunophenotyping to compare wound-associated leukocyte populations between idiopathic and neuromuscular patient groups.
PublicationResveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways
In this study, the authors used the Moxi Go II to quantify lysosomal activation in Lysosomal-METRIQ reporter dermal fibroblasts by measuring GFP and RFP fluorescence and computing a GREEN/RED ratio after resveratrol (and control drug) treatments, providing functional evidence that resveratrol enhances lysosome-dependent degradation pathways.
PublicationButyrolactone I from Aspergillus fungi blocks neutrophil FPR1 to alleviate acute respiratory distress syndrome
The authors used a Moxi cell counter to quantify neutrophil chemotaxis by counting the number of cells that migrated through a 3-µm Boyden-chamber membrane into the lower wells after 90 minutes of fMLF-driven migration (with or without BLI pretreatment).
PublicationSeasonal blood-brain barrier plasticity links environmental cues to migratory behavior in monarch butterflies
The authors used a Moxi GO II to measure viability and concentration of dissociated monarch brain single-cell suspensions immediately before 10X Genomics single-cell capture for scRNA-seq.
PublicationCHD7 binds distinct regions in the Sox11 locus to regulate neuronal differentiation
In this study, the authors used Moxi to standardize plated cell numbers and per-reaction cell inputs for proliferation, neuronal differentiation phenotyping, and genome-wide CHD7 occupancy assays in iMOP progenitors and iMOP-derived neurons.
PublicationConnexin43 hemichannel targeting with TAT-Gap19 alleviates radiation-induced endothelial cell damage
PublicationEfficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions
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