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# Application Notes
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Clear All Resource Type Application Note Instrument Moxi GO II Moxi V Moxi Z Singulator 100 Singulator 200 Singulator 200+ Application Endpoint Counting Immunoprofiling PBMC Analysis Bioprocess Development CAR-T Cell Manufacturing Cell Viability FDA/Regulatory Functional Assay Proliferation & Growth Single Cell Genomics Tissue Dissociation Neuroscience Developmental Biology Drug Discovery FFPE Tissue Immunology Oncology Cardiovascular Organoid Plants Downstream Application 10x Chromium Flex 10x Chromium Single Cell ATAC 10x Chromium Single Cell GEX 10x Chromium Multiome (GEX+ATAC) BioSkryb ResolveDNA BioSpyder TempO-LINC GEX Mission Bio Tapestri Parse Evercode WT Cell or Nuclei Nuclei Cells
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[App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-PBMC-Applications.pdf) Moxi GO II Moxi V Moxi Z
### PBMC Counting + Sizing

Much of the hassle of
analyzing PBMC samples comes from the inadequate counting of the cells of interest, usually because
of debris or lack of robust counting. By circumventing imaging and going back to gold-standard
physics-based principles, every Moxi instrument gives unparalleled abilities in getting the best
cell count. With the added opportunities for fluorescence-based detection with a Moxi V and Moxi GO
II, PBMC viability checks have never been more accurate. Ensure your precious samples aren’t wasted
by making sure you get the quality checks right the first time by relying on a Moxi instrument.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-PBMC-Applications.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Compendium.pdf) Moxi GO II Moxi V Moxi Z
### Applications Compendium

Scientists are concerned with speed,
accuracy, and convenience, and those running the lab and industries are concerned with the cost
typically associated with high-performing instruments. Our proprietary Coulter Principle-based
system delivers on all three accounts, and is therefore a perfect fit for any cell biology benchtop.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Compendium.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Note-Unlocking-the-Next-Stage-in-CAR-T-Cellular-Analysis.pdf) Moxi GO II
### Unlocking the Next Stage in CAR-T Cellular Analysis

There are a variety of ways to monitor this process in cell culture, the two most prominent being
cell counting via imaging-based and coulter-based cell counting methods and higher-level cellular
analysis with fluorophore-tagged antibodies and fluorescent reporters via flow cytometry. While each
instrument used to achieve these two things has its advantages and disadvantages, few and far
between can do both at once. Out of all your options, no other instrument has the power to do both
with the speed, precision, and ease that Precision Cell Systems' Moxi Go II can. In this document,
we will take you through how you can unlock the next stage in your cellular analysis process for
CAR-T with our instruments.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Note-Unlocking-the-Next-Stage-in-CAR-T-Cellular-Analysis.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Single-Cell-Nuclei.pdf) Moxi GO II Moxi V Moxi Z
### Single Cell Nuclei Counting

Protocols for single-cell sequencing library preparation present several challenges along the way
that can impact the accuracy and quality of downstream sequencing results. Making sure you overcome
these is essential for not wasting valuable sample and money on failed sequencing runs. Precision
Cell Systems’ Moxi line of Coulter Principle-based cell analyzers enable you to address all of these
variables in a single instrument, making it the ideal go-to for all of your sequencing needs.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Single-Cell-Nuclei.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Early-Stage-Apoptosis-Monitoring-with-Annexin-V-and-PI.pdf) Moxi GO II
### Early Stage Apoptosis Monitoring

Here, we present the Precision Cell Systems Moxi GO II system as a simple, rapid, and
effective flow cytometric approach to the study of apoptosis using Annexin V
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Early-Stage-Apoptosis-Monitoring-with-Annexin-V-and-PI.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Dual-Stain-Cell-Viability-Assessment.pdf) Moxi GO II Moxi V
### Dual Stain Cell Viability Assessment

The triple-layered cell health assessment where the first layer of isolating cells from debris via
event size, the second layer of PI for staining dead cells, and the third layer of AO for all cells
or calcein for only live cells ensures that every event that is a real cell has been classified as
alive or dead and none are left uncounted. The less layers that are used, the more unreliable the
results. Multiple cell counters on the market are capable of doing this, but the accuracy and
repeatability of data generated by a Moxi Go II makes it the clear winner.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Dual-Stain-Cell-Viability-Assessment.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Cell-Prep-and-Sequencing-Skin-and-Kidney-Cells-with-Parse-Evercode-WT.pdf) Singulator 100 Singulator 200
### Cell Prep and Sequencing Skin and Kidney Cells with Parse Evercode WT

The Singulator platform paired with Parse Biosciences technology enables efficient single-cell
isolation and sequencing from challenging tissue types, with high viability and robust cell
yields suitable for downstream scRNA-seq analysis.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Cell-Prep-and-Sequencing-Skin-and-Kidney-Cells-with-Parse-Evercode-WT.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Cell-Sequencing-of-Tumor-Infiltrating-Lymphocytes-in-Lung-Cancer-with-10x.pdf) Singulator 100 Singulator 200
### Single-Cell Sequencing of Tumor-Infiltrating Lymphocytes in Lung Cancer with 10x

These data demonstrate the ability of the Singulator Platform to prepare single-cell suspensions for
both positive selection by a cell surface marker and single-cell RNA sequencing. These methods
enable researchers to identify genes of interest for potential therapeutic targets for anti-tumor
response
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Cell-Sequencing-of-Tumor-Infiltrating-Lymphocytes-in-Lung-Cancer-with-10x.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Nuclei-Sequencing-of-Mouse-Cerebellum.pdf) Singulator 100 Singulator 200
### Single-Nuclei Sequencing of Mouse Cerebellum with 10x

The Singulator™ Platform provides a reproducible and precise method for isolating nuclei from
complex tissues suitable for single-nuclei RNA sequencing. Our results demonstrate consistent nuclei
yield and high-quality sequencing metrics across biological replicates, making it a reliable tool
for neuroscientific research. The ability to produce high-quality nuclei with minimal variability
enhances the accuracy of downstream snRNA analyses, facilitating deeper insights into normal biology
and disease. Finally, the ability to identify rare cell types underscores the efficacy of the
Singulator Platform in comprehensive cellular analysis, providing a detailed representation of the
cellular landscape.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Nuclei-Sequencing-of-Mouse-Cerebellum.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Singulator-Use-of-Nuclei-Debris-Removal-Reagent.pdf) Singulator 100 Singulator 200
### Singulator Use of Nuclei Debris Removal Reagent

The presence of intracellular and extracellular debris in single-nucleus RNA sequencing can
present a significant challenge in obtaining reliable and accurate results. However, the use of the Precision
Cell Systems Nuclei Debris Removal Reagent can effectively improve the quality of the samples. Our evaluation
of the Nuclei Debris Removal Reagent shows successful removal of debris from traditionally high-debris samples
of brain and liver nuclei while delivering high quality single nuclei suspensions. The resulting samples are
able to be run through microfluidic single nuclei sequencing platforms without issues of clogging or reduced
data quality
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Singulator-Use-of-Nuclei-Debris-Removal-Reagent.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/11/Applicaiton_Note_Singulator_FFPE_v1_11-30-25.pdf) Singulator 200+
### FFPE Tissue Dissociation
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/11/Applicaiton_Note_Singulator_FFPE_v1_11-30-25.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/Research-Spotlight-Optimizing-Nuclei-Isolation-for-Reliable-snRNA-seq-A-Comparative-Study-Validates-the-SingulatorTM-Platform.pdf) Singulator 100 Singulator 200
### Optimizing Nuclei Isolation for Reliable snRNA-seq - A Comparative Study Validates the Singulator Platform
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/Research-Spotlight-Optimizing-Nuclei-Isolation-for-Reliable-snRNA-seq-A-Comparative-Study-Validates-the-SingulatorTM-Platform.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Average-Cell-Size-By-Cell-Type.pdf) Moxi GO II Moxi V Moxi Z
### Moxi - Average Cell Size By Cell Type
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Average-Cell-Size-By-Cell-Type.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Car-T-Activation-Monitoring.pdf) Moxi GO II
### Moxi - CAR-T Activation Monitoring
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Car-T-Activation-Monitoring.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Immunophenotyping-Applications.pdf) Moxi GO II
### Moxi - Immunophenotyping Applications
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Immunophenotyping-Applications.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Monitoring-Cell-Culture-Health-and-MPI-With-the-Moxi-Z.pdf) Moxi Z
### Moxi - Monitoring Cell Culture Health and MPI With the Moxi Z
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Monitoring-Cell-Culture-Health-and-MPI-With-the-Moxi-Z.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Yeast-and-Craft-Beer-Applications.pdf) Moxi GO II Moxi V Moxi Z
### Moxi - Yeast and Craft Beer Applications
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Yeast-and-Craft-Beer-Applications.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Algae-Cell-Counting.pdf) Moxi GO II Moxi V Moxi Z
### Moxi - Algae Cell Counting
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Algae-Cell-Counting.pdf)
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