# Resource Library

Blogs, publications, field guides, ebooks, application notes, and protocols for advanced cell analysis and automated tissue dissociation.

## Featured Collections
[### Cell & Gene Therapy Resources

Explore collection](/resources/collections/cell-gene-therapy/)[### FFPE Resources

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## Browse by Instrument
[Moxi Cell Counters & Flow Cytometry 206 resources](/resources/collections/moxi-resources/) [Moxi V 37](/resources/collections/moxi-resources/?instrument=moxi-v#library)[Moxi Z 77](/resources/collections/moxi-resources/?instrument=moxi-z#library)[Moxi GO II 92](/resources/collections/moxi-resources/?instrument=moxi-go-ii#library) [Singulator Automated Tissue Dissociation 185 resources](/resources/collections/singulator-resources/) [Singulator 100 74](/resources/collections/singulator-resources/?instrument=singulator-100#library)[Singulator 200 70](/resources/collections/singulator-resources/?instrument=singulator-200#library)[Singulator 200+ 41](/resources/collections/singulator-resources/?instrument=singulator-200-plus#library)
## Featured Resources
[Blog](/resources/cold-case-files-brain-tissue-evidence/) Singulator 200+ 2026
### Cold Case - Part 1: The Cold Case Files

Millions of FFPE brain tissue blocks sit in biobanks worldwide, holding decades of evidence about Alzheimer's, Parkinson's, and neurodegenerative diseases. What if we could reopen these cases?
[Read Blog](/resources/cold-case-files-brain-tissue-evidence/) [Field Guide](/resources/minimal-precious-ffpe-samples-singulator-200-plus/) Singulator 200+ 2026
### Working with Minimal and Precious FFPE Samples

Practical strategies for maximizing nuclei recovery from limited FFPE tissue on the Singulator 200+, covering block quality assessment, sectioning waste reduction, the pilot curl approach for irreplaceable specimens, handling needle biopsies and crumbly blocks, and preserving nuclei yield post-processing.
[Read Field Guide](/resources/minimal-precious-ffpe-samples-singulator-200-plus/) [Field Guide](/resources/nuclei-isolation-quality-assessment-yield-viability-dv200-ffpe-singulator/) Singulator 200+ 2026
### FFPE Nuclei Isolation Quality Assessment

Quality assessment of nuclei isolated from FFPE tissue using the Singulator 200+, covering yield measurement, DAPI staining for morphology, DV200 RNA quality metrics, erythrocyte contamination assessment, and structured go/no-go decision frameworks before downstream sequencing.
[Read Field Guide](/resources/nuclei-isolation-quality-assessment-yield-viability-dv200-ffpe-singulator/)
## Full Resource Library

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[Field Guide](/resources/01-invisible-contaminants/)
### Invisible Contaminants

Every cell preparation contains some level of debris—fragments from lysed cells, extracellular matrix remnants, aggregates, and other particulate matter—that quietly skews counts and downstream results.
[Read Field Guide](/resources/01-invisible-contaminants/) [Field Guide](/resources/01-optimization-burden/) Moxi GO II Moxi V
### The Optimization Burden: Hours Wasted on Viability Protocol Development

Every hour spent optimizing viability dye concentrations is an hour not spent on your actual experiments. It's optimized for use - that's the important thing. You don't have to optimize it as a customer. Pre-optimized viability reagents eliminate the titration experiments, the incubation testing, the cell-type-specific protocol development. Why spend time optimizing when validated performance is available from the first use?
[Read Field Guide](/resources/01-optimization-burden/) [Field Guide](/resources/02-unknown-product/) Moxi GO II Moxi V
### The Unknown Product: Most Moxi Users Don't Know This Exists

Most of the issue with viability reagents is that most people don't even know they exist. If you're running viability assays on Moxi V or Moxi GO II with generic dyes you optimized yourself, there's a better option you may not have heard about: pre-optimized, ready-to-use viability reagents designed specifically for your instrument. Now you know.
[Read Field Guide](/resources/02-unknown-product/) [Field Guide](/resources/03-ambient-rna-soup/)
### Ambient RNA Soup

Single-cell genomics platforms capture transcripts from individual cells to reveal heterogeneity that bulk methods miss. That resolution depends on clean single-cell suspensions, where ambient RNA from lysed cells can contaminate every droplet.
[Read Field Guide](/resources/03-ambient-rna-soup/) [Field Guide](/resources/03-dilution-math/)
### The Dilution Math Problem: Every Calculation Is a Potential Error

Consider a simple 1:100 dilution from stock. Errors can enter at multiple points—stock measurement, diluent measurement, mixing adequacy, transfer losses—and each step compounds into measurable variability.
[Read Field Guide](/resources/03-dilution-math/) [Field Guide](/resources/04-compounding-viability-errors/)
### Compounding Viability Errors

Most researchers assume that adding viability stains improves the accuracy of their cell counts. After all, you're adding more information—shouldn't that make the measurement better? The reality is more complicated.
[Read Field Guide](/resources/04-compounding-viability-errors/) [Field Guide](/resources/04-protocol-hunt/) Moxi GO II Moxi V
### The Protocol Hunt: Searching for Methods That Already Exist

Searching for viability protocols, adapting literature methods, trial-and-error until something works - this is time you don't need to spend. The user manual is designed to be super easy. Concentration-based instructions tell you exactly what to do: put X amount of viability reagent and put X amount of sample, incubate and go. No protocol hunting required.
[Read Field Guide](/resources/04-protocol-hunt/) [Field Guide](/resources/05-dissociation-damage-blindspot/)
### The Dissociation Damage Blindspot: Viability Assessment Reveals Protocol Impact

A protocol can achieve high cell yield while producing low cell viability. Aggressive dissociation that frees many cells may simultaneously damage or kill them—a blindspot that viability assessment exposes.
[Read Field Guide](/resources/05-dissociation-damage-blindspot/) [Field Guide](/resources/05-diy-mentality/) Moxi GO II Moxi V
### The DIY Mentality: When Making Your Own Viability Reagent No Longer Makes Sense

That's why people - including me - just buy premixed gel loading dye and don't make my own from powder like my PI wanted me to. The same logic applies to viability reagents. Buy the damn gels rather than making them - consistency and data. When convenience and consistency matter more than tradition, pre-made beats DIY.
[Read Field Guide](/resources/05-diy-mentality/) [Field Guide](/resources/05-missing-qc-checkpoint/)
### Missing QC Checkpoints

Every laboratory has protocols for cell culture, staining, and instrument operation. But ask about sample quality standards—specifically, debris thresholds—and you'll often find a blind spot where a QC checkpoint should be.
[Read Field Guide](/resources/05-missing-qc-checkpoint/) [Field Guide](/resources/05-suboptimal-resolution/) Moxi GO II Moxi V Moxi Z
### The Suboptimal Resolution Problem: Why Your Cell Populations Look Merged

Using an aperture much larger than necessary reduces sizing resolution by creating smaller signal differences between cell sizes. Cell populations that should be distinguishable appear merged when the aperture is too large for the cells being measured. Target 15-40% of aperture diameter for optimal resolution. If you're counting lymphocytes on M+ cassettes because it works, you're sacrificing the sizing resolution that S+ cassettes would provide.
[Read Field Guide](/resources/05-suboptimal-resolution/) [Field Guide](/resources/06-batch-consistency/) Moxi GO II Moxi V
### Viability Reagent Batch-to-Batch Consistency: Why Your Results Vary Over Time

When you make your own viability reagents, every batch is different. When you buy pre-optimized reagents with QC'd lot consistency, every lot performs the same. Long-term experiments need long-term consistency - and that consistency comes from manufacturing quality control, not from hoping your technique stays identical over months of work.
[Read Field Guide](/resources/06-batch-consistency/) [Field Guide](/resources/06-fifteen-micron-boundary/) Moxi GO II Moxi V Moxi Z
### The 15-Micrometer Decision: A Practical Cassette Selection Framework

The 15 μm boundary provides clear selection criterion: cells under 15 micrometers use S+ cassettes, cells over 15 micrometers use M+ cassettes. This boundary isn't arbitrary - it's where each aperture size achieves the optimal 15-40% cell-to-aperture ratio for signal quality and sizing resolution. Know your cell size, follow the boundary, and cassette selection becomes automatic.
[Read Field Guide](/resources/06-fifteen-micron-boundary/) [Field Guide](/resources/06-physics-vs-algorithms/)
### Physics vs Algorithms

Every AI-based image counter was trained on a specific dataset, learning to recognize "cell" and "not cell" from images someone curated. When your sample doesn't match that training set, physics-based counting tells a more reliable story.
[Read Field Guide](/resources/06-physics-vs-algorithms/) [Field Guide](/resources/06-singulator-integration-workflow/)
### Singulator Integration Workflow: Your Sous Chef for Automated Tissue Dissociation

The Precision Cell Systems Singulator provides automated, standardized tissue dissociation. But automation doesn't mean zero debris or guaranteed sample quality—integrating a Moxi QC checkpoint is what makes the workflow dependable.
[Read Field Guide](/resources/06-singulator-integration-workflow/) [Field Guide](/resources/07-preloading-qc-checkpoint/)
### Preloading QC Checkpoints

Single-cell genomics workflows have a critical decision point: do you load this sample onto an expensive chip, or does it need more cleanup first? A preloading QC checkpoint answers that with confidence.
[Read Field Guide](/resources/07-preloading-qc-checkpoint/) [Field Guide](/resources/07-til-immune-killing-optimization/) Moxi GO II Moxi V Moxi Z
### TIL Counting and Immune Cell Killing: Dual-Cassette Optimization Guide

TIL counting and immune cell killing assays are immediate applications for dual-cassette workflows. Cancer cells are large, T cells are small - no single cassette optimizes both. Run S+ for accurate T cell counts, M+ for accurate tumor/target counts. The extra run takes minutes but delivers publication-quality E:T ratios and killing percentages.
[Read Field Guide](/resources/07-til-immune-killing-optimization/) [Field Guide](/resources/07-training-new-users/) Moxi GO II Moxi V
### Training New Users: Why Pre-Optimized Reagents Simplify Onboarding

New lab members need to generate valid data quickly. Teaching protocol optimization takes weeks. Teaching protocol execution takes minutes. The user manual is designed to be super easy - put X amount of viability reagent, put X amount of sample, incubate and go. When reagents are pre-optimized, training focuses on execution, not development.
[Read Field Guide](/resources/07-training-new-users/) [Field Guide](/resources/01-small-cell-weak-signal/) Moxi GO II Moxi V Moxi Z
### The Small Cell Weak Signal Problem: Why Your Lymphocyte Counts May Be Wrong

Small cells measured through oversized apertures generate weak electrical signals that fall below detection thresholds or get confused with debris. If you're counting lymphocytes, PBMCs, Jurkat cells, or any suspension lines under 15 micrometers with the wrong cassette, you're likely undercounting. Switch to S+ cassettes where the smaller aperture ensures your small cells generate strong, detectable signals clearly distinguishable from noise.
[Read Field Guide](/resources/01-small-cell-weak-signal/) [Field Guide](/resources/02-large-cell-clog-risk/) Moxi GO II Moxi V Moxi Z
### The Large Cell Clog Risk: Preventing Measurement Interruptions

Large cells approaching the aperture diameter create artificially high signals and risk clogging the sensing orifice. Clogging interrupts runs, wastes samples, and requires cassette replacement mid-experiment. For adherent cell lines like CHO, HEK293, and HeLa, and for primary tissue cells over 15 micrometers, M+ cassettes provide the larger aperture necessary to prevent physical blockage.
[Read Field Guide](/resources/02-large-cell-clog-risk/) [Field Guide](/resources/03-mixed-population-dilemma/) Moxi GO II Moxi V Moxi Z
### The Mixed Population Dilemma: When One Cassette Can't Capture Everything

When your sample contains both small and large cells, no single cassette optimizes measurement for both populations. The solution: run the same sample twice - once with S+ to get accurate small cell counts, once with M+ to get accurate large cell counts. This dual-cassette workflow delivers accurate data for both populations rather than compromised data for everyone.
[Read Field Guide](/resources/03-mixed-population-dilemma/) [Field Guide](/resources/04-coincidence-artifact/) Moxi GO II Moxi V Moxi Z
### The Coincidence Artifact: When Two Cells Count as One

Coincidence - multiple cells in the aperture simultaneously - causes two cells to be counted as one, corrupting both count and size data. Optimal aperture utilization means targeting 15-40% of aperture diameter so cells generate strong signals while avoiding coincidence artifacts. Match your cassette to your cell size, stay within concentration guidelines, and coincidence becomes a non-issue.
[Read Field Guide](/resources/04-coincidence-artifact/) [Field Guide](/resources/brain-tissue-complexity-myelin-lipids-neuronal-nuclei/) Singulator 200+
### Overcoming Brain Tissue Complexity: Myelin, Lipids, and Fragile Neuronal Nuclei

Brain FFPE tissue creates unique nuclei isolation challenges. Myelin debris, lipid contamination, and fragile neuronal nuclei require controlled automated processing to preserve cell-type diversity for single-nucleus sequencing.
[Read Field Guide](/resources/brain-tissue-complexity-myelin-lipids-neuronal-nuclei/) [Field Guide](/resources/brain-tumor-ffpe-surgical-resection-single-cell/) Singulator 200+
### Brain Tumor FFPE Processing: From Surgical Resection to Single-Nucleus Insights

Process brain tumor FFPE from surgical resections on the Singulator 200+. Preserve cancer cells and immune populations for snRNA-seq and spatial analysis.
[Read Field Guide](/resources/brain-tumor-ffpe-surgical-resection-single-cell/) [Ebook](/resources/cold-case-files-neuroscience-ffpe/) Singulator 200+
### Cold Case Files:The Brain's Embedded Evidence

How the Singulator 200+ preserves fragile neuronal nuclei from irreplaceable postmortem brain tissue. Automated FFPE processing for Alzheimer's, brain tumors, and brain atlases.
[Read Ebook](/resources/cold-case-files-neuroscience-ffpe/) [Field Guide](/resources/ffpe-neurodegenerative-disease-research-singulator/) Singulator 200+
### FFPE Nuclei Extraction for Neurodegenerative Disease Research

Extract nuclei from FFPE brain tissue for Alzheimer's, Parkinson's, and Lewy body research. Longitudinal cohorts, cell-type preservation, and disease staging on the Singulator 200+.
[Read Field Guide](/resources/ffpe-neurodegenerative-disease-research-singulator/) [Field Guide](/resources/integrating-snrna-seq-spatial-transcriptomics-brain-ffpe/) Singulator 200+
### Integrating snRNA-seq with spatial transcriptomics for brain mapping

Pair spatial transcriptomics with snRNA-seq from the same FFPE brain block. Block allocation, platform selection, and nuclei quality for multi-omic brain studies.
[Read Field Guide](/resources/integrating-snrna-seq-spatial-transcriptomics-brain-ffpe/) [Field Guide](/resources/maximizing-limited-brain-tissue-sections-ffpe/) Singulator 200+
### Maximizing Results from Limited Brain Tissue Sections

Get high-quality nuclei from limited postmortem brain FFPE sections. Process NIH biobank allocations, hospital archives, and surgical specimens on the Singulator 200+.
[Read Field Guide](/resources/maximizing-limited-brain-tissue-sections-ffpe/) [Field Guide](/resources/navigating-postmortem-brain-tissue-ffpe-genomics/) Singulator 200+
### Navigating postmortem brain tissue for FFPE genomics

Practical guide to postmortem brain FFPE challenges: necropsy timing, fixation variability, biobank sourcing, myelin debris, and how the Singulator 200+ standardizes nuclei extraction.
[Read Field Guide](/resources/navigating-postmortem-brain-tissue-ffpe-genomics/) [Field Guide](/resources/reproducible-multi-site-brain-tissue-atlases-singulator/) Singulator 200+
### Building Reproducible Multi-Site Brain Tissue Atlases

Standardize brain FFPE nuclei extraction across consortium sites with the Singulator 200+. Eliminate operator variability, prevent batch effects, and scale for atlas projects.
[Read Field Guide](/resources/reproducible-multi-site-brain-tissue-atlases-singulator/) [Webinar](/resources/unlocking-single-cell-insights-from-ffpe-samples/) Singulator 200+
### Unlocking Single-Cell Insights from FFPE Samples

Ronan Chaugnet from Memorial Sloan Kettering shares several years of head-to-head benchmarking data comparing nuclei extraction platforms for FFPE tissue. The Singulator 200+ and Miltenyi GentleMACS both outperformed manual approaches across glioblastoma, liver, lung, pancreas, and tumor samples, with the Singulator 200+ standing out for its fully automated, enzyme-free workflow. Ronan also walks through three methods his lab has built on top of automated FFPE extraction: 10X Genomics FLEX for high-quality gene expression, PERFF-Seq for sorting rare cell populations by RNA markers, and GIFT-Seq for detecting 600+ mutations at single-cell resolution from archival tissue.
[Read More](/resources/unlocking-single-cell-insights-from-ffpe-samples/) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Automating-Nuclei-Isolation-from-FFPE-Tissues-for-Single-Nuclei-Sequencing-Applications.pdf) Singulator 200+
### Automating Nuclei Isolation from FFPE Tissues for Single-Nuclei Sequencing Applications

This protocol describes how to isolate, count, and prepare single nuclei from FFPE (formalin-fixed, paraffin-embedded) tissues for single-nuclei sequencing assays using the Singulator 200+ platform. Deparaffinization and rehydration of the tissue using solvent and various concentrations of ethanol are conducted on the Singulator 200+ platform along with automated nuclei isolation.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Automating-Nuclei-Isolation-from-FFPE-Tissues-for-Single-Nuclei-Sequencing-Applications.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-V-Nuclei-Counting-Protocol.pdf) Moxi V
### Nuclei Counting Protocol

Nuclei counting protocol for Moxi V
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-V-Nuclei-Counting-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-V-Viability-Staining-PI-Protocol.pdf) Moxi V
### Viability Staining (PI) Protocol

Viability staining protocol for Moxi V
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-V-Viability-Staining-PI-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-V-Validation-Check-Bead-Procedure.pdf) Moxi V
### Validation Check Bead Procedure

Validation check protocol for Moxi V
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-V-Validation-Check-Bead-Procedure.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-Z-Total-WBC-Count-Protocol.pdf) Moxi Z
### Total WBC Count Protocol

WBC Counting Protocol for Moxi Z
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-Z-Total-WBC-Count-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Nuclei-Nucleated-Cell-Counting-with-Coulter-Principle-and-PI.pdf) Moxi GO II
### Nucleated Cell Counting with Coulter Principle and PI

This protocol describes an approach to achieve rapid, highly-accurate nuclei / nucleated-cell counts by first lysing the cells to isolate the nuclei, labeling the nuclei with PCS Viability Reagent, and counting using the Moxi GO II system
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Nuclei-Nucleated-Cell-Counting-with-Coulter-Principle-and-PI.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-Z-System-Check-Bead-Protocol.pdf) Moxi Z
### System Check Bead Protocol

System Check Bead Protocol for Moxi Z
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-Z-System-Check-Bead-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0004-PCS-Demonstrated-Protocol-nuclei-isolation-from-FFPE-v241118.pdf) Singulator 100 Singulator 200
### Nuclei extraction from FFPE

This protocol describes how to isolate, count, and prepare single nuclei from FFPE (formalin-fixed,paraffin-embedded) tissues for single-nuclei sequencing assays using the Singulator Platform.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0004-PCS-Demonstrated-Protocol-nuclei-isolation-from-FFPE-v241118.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi_GO_II_-_Acridine_Orange_AOJ_nucleated_cells_and_Viability_PI_-_Staining_Protocol.pdf) Moxi GO II
### Acridine Orange (AO) and PI Staining Protocol

This protocol describes how to stain with both propidium iodide and acridine orange. It also explains how to use compensation settings on the Moxi GO II
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi_GO_II_-_Acridine_Orange_AOJ_nucleated_cells_and_Viability_PI_-_Staining_Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Apoptosis-FITC-Annexin-V-and-Viability-PI-Staining-Protocol.pdf) Moxi GO II
### Apoptosis (FITC-Annexin V) and Viability (PI) - Staining Protocol

This protocol outlines a standardized dual-staining workflow for the Moxi GO II to simultaneously quantify apoptosis and viability using FITC-Annexin V and concentrated PCS Viability Reagent.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Apoptosis-FITC-Annexin-V-and-Viability-PI-Staining-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Cell-Health-Calcein-AM-and-Viability-PI-Staining-Protocol.pdf) Moxi GO II
### Cell Health (Calcein-AM) and Viability (PI) - Staining Protocol

This protocol outlines a dual-staining procedure using Calcein-AM and Propidium Iodide on the Moxi GO II to simultaneously assess cellular membrane integrity and metabolic vitality for a comprehensive evaluation of cell health.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Cell-Health-Calcein-AM-and-Viability-PI-Staining-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Cell-Surface-Immunolabeling-Staining-Protocol.pdf) Moxi GO II
### Cell Surface Immunolabeling - Staining Protocol

This protocol details the standardized preparation, blocking, and immunolabeling of single-cell suspensions using either direct or secondary antibody staining workflows optimized for high-resolution population analysis on the Moxi GO II flow cytometer.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Cell-Surface-Immunolabeling-Staining-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0001-PCS-Cell-Isolation-from-Intestine-Tissue-for-Single-Cell-Sequencing-Applications-Demonstrated-Protocol.pdf) Singulator 100 Singulator 200
### Cell Isolation from Intestine Tissue for Single Cell Sequencing Applications Demonstrated Protocol

This protocol outlines the process for isolating, cleaning, counting, and preparing single cells fromfresh intestine tissue for single-cell sequencing assays listed below. Some
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0001-PCS-Cell-Isolation-from-Intestine-Tissue-for-Single-Cell-Sequencing-Applications-Demonstrated-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0002-PCS-Demonstrated-Protocol-Cell-Isolation-from-Fresh-Tissues-v241218.pdf) Singulator 100 Singulator 200
### Cell Isolation from Fresh Tissues Demonstrated Protocol

This protocol outlines the process for isolating, cleaning, counting, and preparing single cells from fresh tissue for the single-cell sequencing assays listed below
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0002-PCS-Demonstrated-Protocol-Cell-Isolation-from-Fresh-Tissues-v241218.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0003-PCS-genomics-Demonstrated-Protocol-FFPE-and-PFA-fixed-Tissues-Cells-v241206B.pdf) Singulator 100 Singulator 200
### Demonstrated-Protocol-FFPE and PFA fixed Tissues-Cells

This protocol outlines the steps for isolating, counting, and preparing single cells from FFPE (formalin-fixed,paraffin-embedded) or paraformaldehyde (PFA) fixed whole tissues for single-cell sequencing assays using theSingulator™ Platform. The process includes automated deparaffinization, rehydration, and dissociation.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0003-PCS-genomics-Demonstrated-Protocol-FFPE-and-PFA-fixed-Tissues-Cells-v241206B.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0006-PCS-genomics-demonstrated-protocol-nuclei-isolation-and-cleanup-frozen-mouse-brain-v241018.pdf) Singulator 100 Singulator 200
### demonstrated-protocol-nuclei-isolation-and-cleanup-frozen-mouse-brain-v241018

This protocol describes how to isolate, clean, count, and prepare single nuclei from fresh frozenmouse brain tissue for single-nuclei sequencing applications. Some optimizations of theSingulator™ protocol parameters may be needed based on storage time and tissue condition.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0006-PCS-genomics-demonstrated-protocol-nuclei-isolation-and-cleanup-frozen-mouse-brain-v241018.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0005-PCS-genomics-Demonstrated-Protocol-nuclei-isolation-and-cleanup-from-frozen-tissue-v241106v2.pdf) Singulator 100 Singulator 200
### Demonstrated-Protocol-nuclei-isolation-and-cleanup-from-frozen-tissue-v241106v2

This protocol describes how to isolate, clean, count, and preparesingle nuclei from frozen tissue for single-nuclei sequencing assays.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/DP0005-PCS-genomics-Demonstrated-Protocol-nuclei-isolation-and-cleanup-from-frozen-tissue-v241106v2.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Validation-Check-Bead-Procedure.pdf) Moxi GO II
### Validation Check Bead Procedure

This protocol provides step-by-step instructions for preparing, running, and gating validation check beads to verify the sizing, concentration, and fluorescence accuracy of the Moxi GO II instrument
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Validation-Check-Bead-Procedure.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Whole-Blood-Intracellular-Immunolabeling-Staining.pdf) Moxi GO II
### Whole Blood - Intracellular Immunolabeling Staining

This document provides a detailed protocol for isolating, stimulating, fixing, permeabilizing, and intracellularly immunolabeling white blood cells from whole blood samples for analysis on the Moxi GO II.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Whole-Blood-Intracellular-Immunolabeling-Staining.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Oxidative-Stress-Protocol.pdf) Moxi GO II
### Oxidative Stress Protocol

This document provides a step-by-step protocol for measuring oxidative stress in living cells by detecting reactive oxygen species (ROS) using the CellROX Green assay on the Moxi GO II.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-Oxidative-Stress-Protocol.pdf) [Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-RBC-Lysis-Cell-Surface-Immunolabeling-Protocol.pdf) Moxi GO II
### RBC Lysis - Cell Surface Immunolabeling Protocol

This document provides a step-by-step protocol for immunolabeling the surface of white blood cells while effectively eliminating red blood cell interference through lysis and optional buffy coat isolation for analysis on the Moxi GO II.
[Read Protocol](https://precisioncellsystems.com/wp-content/uploads/2026/03/Moxi-GO-II-RBC-Lysis-Cell-Surface-Immunolabeling-Protocol.pdf) [Blog Part 5](/resources/cold-case-closed-brain-atlas/) Singulator 200+
### Cold Case - Part 5: Case Closed

Brain atlases are being built. Archival tissue is finally talking. How standardized nuclei extraction and platform-agnostic analysis are solving neuroscience's oldest cold cases.
[Read Blog](/resources/cold-case-closed-brain-atlas/) [Blog Part 2](/resources/cold-case-contaminated-evidence-brain-tissue/) Singulator 200+
### Cold Case - Part 2: Contaminated Evidence

Manual extraction of nuclei from FFPE brain tissue destroys 50 to 60 percent of starting material. Fragile neurons die first, leaving biased results. Here is what goes wrong.
[Read Blog](/resources/cold-case-contaminated-evidence-brain-tissue/) [Blog Part 1](/resources/cold-case-files-brain-tissue-evidence/) Singulator 200+
### Cold Case - Part 1: The Cold Case Files

Millions of FFPE brain tissue blocks sit in biobanks worldwide, holding decades of evidence about Alzheimer's, Parkinson's, and neurodegenerative diseases. What if we could reopen these cases?
[Read Blog](/resources/cold-case-files-brain-tissue-evidence/) [Blog Part 4](/resources/cold-case-forensic-lab-singulator-200-plus/) Singulator 200+
### Cold Case - Part 4: The Forensic Lab

Manual FFPE processing destroys fragile neuronal nuclei and produces variable results. The Singulator 200+ automates the workflow with a two-cartridge system that delivers consistent, operator-independent results from irreplaceable brain tissue.
[Read Blog](/resources/cold-case-forensic-lab-singulator-200-plus/) [Blog Part 3](/resources/cold-case-one-shot-at-the-scene/) Singulator 200+
### Cold Case - Part 3: One Shot at the Scene

NIH biobanks give you one allocation of irreplaceable brain tissue. Manual processing destroys 50-60% before analysis begins. The extraction method is the real variable.
[Read Blog](/resources/cold-case-one-shot-at-the-scene/) [Field Guide](/resources/complete-ffpe-workflow-archive-to-publication-singulator/) Singulator 200+
### Complete FFPE workflow: from archive to publication with the Singulator 200+

End-to-end protocol walkthrough from FFPE block selection through deparaffinization, nuclei isolation, quality control, library preparation, sequencing, and data analysis using the Singulator 200+.
[Read Field Guide](/resources/complete-ffpe-workflow-archive-to-publication-singulator/) [Field Guide](/resources/deparaffinization-rehydration-singulator-200-plus-green-cartridge/) Singulator 200+
### Deparaffinization Rehydration Optimization

How the Singulator 200+ GREEN cartridge automates FFPE deparaffinization and rehydration, eliminating toxic solvents, fume hoods, and manual ethanol series from nuclei extraction workflows.
[Read Field Guide](/resources/deparaffinization-rehydration-singulator-200-plus-green-cartridge/) [Field Guide](/resources/ffpe-downstream-platform-compatibility/) Singulator 200+
### FFPE Downstream Platform Compatibility

Practical guide to connecting Singulator 200+ FFPE nuclei with downstream analysis platforms including 10x Chromium Flex, Xenium, ATAC-seq, Visium, and MERFISH, covering quality requirements, expected yields, and multi-platform study design.
[Read Field Guide](/resources/ffpe-downstream-platform-compatibility/) [Field Guide](/resources/ffpe-input-preparation-optimizing-curl-thickness-tissue-mass-block-age-singulator-200-plus/) Singulator 200+
### FFPE Input Preparation

Practical guide to preparing FFPE tissue inputs for the Singulator 200+ automated nuclei extraction platform, covering curl thickness selection, tissue mass requirements, block age effects, quality assessment, and handling difficult or precious specimens.
[Read Field Guide](/resources/ffpe-input-preparation-optimizing-curl-thickness-tissue-mass-block-age-singulator-200-plus/) [Field Guide](/resources/ffpe-troubleshooting-guide-singulator-200-plus/) Singulator 200+
### FFPE Troubleshooting Guide: Diagnosing and Resolving Common Singulator 200+ Issues

Practical troubleshooting guide for the Singulator 200+ FFPE nuclei extraction workflow, covering the five most common problem categories: low yield, excessive debris, poor RNA quality, batch-to-batch variability, and cartridge/instrument errors.
[Read Field Guide](/resources/ffpe-troubleshooting-guide-singulator-200-plus/) [Field Guide](/resources/minimal-precious-ffpe-samples-singulator-200-plus/) Singulator 200+
### Working with Minimal and Precious FFPE Samples

Practical strategies for maximizing nuclei recovery from limited FFPE tissue on the Singulator 200+, covering block quality assessment, sectioning waste reduction, the pilot curl approach for irreplaceable specimens, handling needle biopsies and crumbly blocks, and preserving nuclei yield post-processing.
[Read Field Guide](/resources/minimal-precious-ffpe-samples-singulator-200-plus/) [Field Guide](/resources/nuclei-isolation-quality-assessment-yield-viability-dv200-ffpe-singulator/) Singulator 200+
### FFPE Nuclei Isolation Quality Assessment

Quality assessment of nuclei isolated from FFPE tissue using the Singulator 200+, covering yield measurement, DAPI staining for morphology, DV200 RNA quality metrics, erythrocyte contamination assessment, and structured go/no-go decision frameworks before downstream sequencing.
[Read Field Guide](/resources/nuclei-isolation-quality-assessment-yield-viability-dv200-ffpe-singulator/) [Ebook](/resources/tissue-dissociation-protocol-guide/) Singulator 100 Singulator 200 Singulator 200+
### Tissue Dissociation Guide

The most comprehensive tissue dissociation reference available. Interactive protocols for single-cell isolation and nuclei extraction across 57+ tissue types with community and Singulator-optimized methods.
[Read Ebook](/resources/tissue-dissociation-protocol-guide/) [Ebook](/resources/unlock-the-archive-ffpe-nuclei-extraction/) Singulator 200+
### Unlock the Archive: Automated FFPE Nuclei Extraction for Single-Cell Genomics

How formalin-fixed paraffin-embedded tissue archives hold enormous potential for single-cell genomics, and how the Singulator 200+ automates the nuclei extraction workflow to unlock that potential
[Read Ebook](/resources/unlock-the-archive-ffpe-nuclei-extraction/) [Ebook](/resources/your-cell-count-is-a-safeguard/) Moxi GO II Moxi V Moxi Z
### Your Cell Count is a Safeguard

The debris problem: Membrane fragments, aggregates, media residue, and lysed cell debris are present in virtually every biological preparation. How does your counting method distinguish a cell from a piece of debris?
[Read Ebook](/resources/your-cell-count-is-a-safeguard/) [Publication](https://www.cell.com/cell-stem-cell/pdfExtended/S1934-5909(25)00367-4) Moxi V
### Human heart-macrophage assembloids mimic immune-cardiac interactions and enable arrhythmia disease modeling
[View Publication](https://www.cell.com/cell-stem-cell/pdfExtended/S1934-5909(25)00367-4) [Publication](https://www.mdpi.com/1420-3049/30/3/629) Moxi Z
### Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells

In this study, the authors used a Moxi Z Cell Counter to count trypsinized, treated PANC-1 cells so they could re-plate defined cell numbers for colony formation assays and accurately calculate plating efficiency and survival fraction after PAA-TiOx nanoparticle and X-ray treatments under normoxic and hypoxic conditions.
[View Publication](https://www.mdpi.com/1420-3049/30/3/629) [Publication](https://pubmed.ncbi.nlm.nih.gov/41345064/) Moxi Z
### Synthesis and Application of Nanovanilloids for Localized Therapeutic Hypothermia after Traumatic Brain Injury

The authors used a Moxi Z to count cells as part of preparing mammalian cell cultures for in vitro nanovanilloid testing (viability/oxidative-stress assays and TRPV1 calcium-influx imaging).
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41345064/) [Publication](https://www.nature.com/articles/s42003-025-07753-1?utm_source=chatgpt.com) Moxi GO II
### ARNT-dependent HIF-2α signaling protects cardiac microvascular barrier integrity and heart function post-myocardial infarction

In this study, the authors used the Moxi GO II to quantify endothelial apoptosis after Hif2α siRNA knockdown and hypoxic exposure, using Annexin V–FITC and PI with the instrument’s apoptosis app to measure cell-death outcomes under their ischemia-relevant conditions.
[View Publication](https://www.nature.com/articles/s42003-025-07753-1?utm_source=chatgpt.com) [Publication](https://pubmed.ncbi.nlm.nih.gov/40504625/) Moxi GO II
### Device-free isolation of photoreceptor cells from patient iPSC-derived retinal organoids

In your staged partial-dissociation retinal organoid workflow, you used the Moxi Go II to count the filtered dissociation-fraction cell suspensions immediately before 10x Chromium X scRNA-seq loading (targeting ~8,000 cells per sample), enabling consistent single-cell capture across fractions and lines.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40504625/) [Publication](https://www.biorxiv.org/content/10.1101/2025.11.18.688525v2) Moxi V
### Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms

The authors used a Moxi V to measure Aspergillus niger spore concentration, enabling per-10^6-spore normalization of downstream plate-reader GFP fluorescence when comparing transformants across binary vector ORI variants.
[View Publication](https://www.biorxiv.org/content/10.1101/2025.11.18.688525v2) [Publication](https://pubmed.ncbi.nlm.nih.gov/40128410/) Moxi Z
### Induction of a transcriptional adaptation response by RNA destabilization events

The authors used the MOXI Z Mini to count and standardize 50,000 NIH3T3 cells per condition immediately before ATAC-seq library preparation, supporting their chromatin-accessibility comparisons at the Actg2 locus across RNA-destabilizing perturbations.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40128410/) [Publication](https://pubmed.ncbi.nlm.nih.gov/41398915/) Moxi Z
### Transcriptome profiling of peripheral blood mononuclear cells from highly susceptible adult cattle infected with a virulent strain of Babesia bovis

The authors used a Moxi Z to count isolated bovine PBMCs after density separation and washes, then normalized each sample to ~2×10^7 cells per tube prior to RNAlater stabilization and downstream bulk RNA-Seq.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41398915/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40564967/) Moxi Z
### Mitochondrial Transfer from Human Platelets to Rat Dental Pulp-Derived Fibroblasts in the 2D In Vitro System: Additional Implication in PRP Therapy

The author used a platelet–fibroblast co-culture model, and a Moxi-Z Coulter-based cell counter to quantify RPC-C2A fibroblast cell numbers after UVC injury and platelet/lysate treatment and to normalize fibroblast-associated ATP measurements to cell count, supporting your survival and bioenergetic conclusions.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40564967/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40778478/) Moxi GO II
### Secretion of specific metabolites and changes in miRNA expression in murine osteoblastic cells exposed in vitro to α-radiation

The authors used a Moxi GO II to perform fluorescence-based flow cytometric phenotyping of BM-derived cultures (CD45 and intracellular RUNX2/BAP) to verify the osteoblastic differentiation context for interpreting downstream α-radiation–responsive omics measurements.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40778478/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40282462/) Moxi GO II
### 5-Aminolevulinic Acid: A Novel Approach to Improving Radioresistance in Prostate Cancer

In this study, the authors used the Moxi GO II to run a FITC-based apoptosis assay on treated prostate cancer cells (±5-ALA, ±irradiation) and quantify early apoptotic fractions as a mechanistic readout of radiosensitization.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40282462/) [Publication](https://www.biorxiv.org/content/10.64898/2025.12.22.695993v1) Moxi Z
### The transcription of a single olfactory receptor per neuron is enforced by epigenetic silencing of their enhancers

The authors used a Moxi cell counter (Orflo, S cassettes) to quantify the live, post-FACS-sorted MOE single-cell suspension and dilute it to ~1000 cells/µL for 10x Genomics single-cell RNA-seq loading.
[View Publication](https://www.biorxiv.org/content/10.64898/2025.12.22.695993v1) [Publication](https://pubmed.ncbi.nlm.nih.gov/41368071/) Moxi GO II Moxi Z
### Proliferation of a bloom-forming phytoplankton via uptake of polyphosphate-accumulating bacteria under phosphate-limiting conditions

In this study, the authors used a Moxi Z to quantify H. akashiwo cell-density changes in phosphate-depleted co-cultures and a Moxi Go II Mini to measure CellTracker™ Green–labeled bacterial fluorescence associated with algal-sized, red-autofluorescent particles as evidence of bacterivory linked to growth rescue under phosphate limitation.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41368071/) [Publication](https://pubmed.ncbi.nlm.nih.gov/39694029/) Moxi Z
### Tubulin sequence divergence is associated with the use of distinct microtubule regulators

In this study of Naegleria’s mitotic vs differentiation microtubule programs, the authors used a Moxi Z impedance-based counter to quantify amoeba concentration so they could inoculate and grow cultures at defined densities before initiating the mitotic synchronization workflow.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/39694029/) [Publication](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0323136) Moxi GO II
### Small-scale protocols to characterize mitochondrial Complex V activity and assembly in peripheral blood mononuclear cells

In this study, the authors used a Moxi GO II (561 nm LP filter) to quantify TMRM fluorescence in PBMCs and calculate inhibitor-dependent shifts in the fraction of TMRM-positive cells as a small-sample, intact-cell readout of mitochondrial membrane potential (∆Ѱm) and Complex V operating mode.
[View Publication](https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0323136) [Publication](https://www.biorxiv.org/content/10.64898/2025.12.24.696225v1) Moxi Z
### Laser-driven VHEE pulsed fast fractionation (PFF): second-scale inter-pulse timing differentially modulates normal tissue and tumour toxicity

The authors used an ORFLO Moxi Mini to count viable cells after irradiated MRC5-hTERT and HCT116 pellets were re-plated and expanded for 5–6 days, using the averaged viable-cell counts (normalized to same-day non-irradiated controls) as the viability endpoint to compare inter-pulse timing conditions.
[View Publication](https://www.biorxiv.org/content/10.64898/2025.12.24.696225v1) [Publication](https://pubmed.ncbi.nlm.nih.gov/40867841/) Moxi Z
### Selective Inhibition of Vascular Smooth Muscle Cell Function by COVID-19 Antiviral Drugs: Impact of Heme Oxygenase-1

The authors used a Moxi Z to count trypsinized vascular smooth muscle cells after a 3-day antiviral treatment, providing the primary quantitative endpoint for proliferation in their remdesivir vs. molnupiravir/nirmatrelvir comparisons.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40867841/) [Publication](https://pubmed.ncbi.nlm.nih.gov/41221847/) Moxi Z
### Bmpr2 Loss in the Vascular Endothelium Enhances Neoangiogenesis and Growth of Lung Metastatic Lesions in a Mouse Model of Breast Cancer

The authors used a Moxi Z (Type-M cassettes) to collect 24-hour interval EO771 cell counts and build proliferation curves to test whether BMP9 treatment or BMPR2-silenced endothelial conditioned media/ECM altered EO771 growth.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41221847/) [Publication](https://pubmed.ncbi.nlm.nih.gov/39797760/) Moxi GO II
### NKX2. 2 and KLF4 cooperate to regulate α-cell identity

In this study, the authors used a Moxi GO II with propidium iodide to measure cell count and viability of αTC cells after Klf4 siRNA transfection, supporting the subsequent qPCR-based knockdown validation and selection of knockdown samples for downstream mechanistic assays.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/39797760/) [Publication](https://pubmed.ncbi.nlm.nih.gov/39949927/) Moxi GO II
### Tumor-treating fields and concurrent cisplatin: an in vitro demonstration of efficacy in triple-negative breast cancer

In this TNBC cisplatin in vitro study, the authors used the Moxi GO II to run an Annexin V (FITC) / PI apoptosis flow-cytometry assay (and to capture 24-hour flow-based cell-count outcomes) to quantitatively compare treatment-induced killing in TNBC versus epithelial cells across mono-culture and co-culture conditions.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/39949927/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40147445/) Moxi GO II
### A patient-derived T cell lymphoma biorepository uncovers pathogenetic mechanisms and host-related therapeutic vulnerabilities

The authors used a Moxi GO II to count and monitor CAR T cells (including cell size/growth kinetics) throughout expansion, advancing the products into in vitro cytotoxicity assays and in vivo PTCL PDX studies only once the cells’ kinetics/size indicated they had “rested” from stimulation.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40147445/) [Publication](https://www.sciencedirect.com/science/article/abs/pii/S1385894726004341) Moxi Z
### Investigating Nanoparticle Distribution and Tissue Architecture in Male and Female Human Cardiac Organoids

The authors used a Moxi cell counter to accurately seed 10,000 iPSCs per well when generating human cardiac organoids, ensuring consistent starting organoid inputs before assessing fluorescent nanoparticle biodistribution and cell-type–specific uptake by downstream fluorescence microscopy.
[View Publication](https://www.sciencedirect.com/science/article/abs/pii/S1385894726004341) [Publication](https://pubmed.ncbi.nlm.nih.gov/41214305/) Moxi Z
### Pulmonary Vascular Endothelial Dysfunction Is Induced by Nonpulsatile Pulmonary Blood Flow in an Ovine Classic Glenn Model

The authors used a Moxi Z to count primary pulmonary artery endothelial cells after trypsinization—both to seed 6000 cells/well and to perform serial 24-hour cell counts through 120 hours—to quantify and compare PAEC proliferation between control and Glenn lambs.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41214305/) [Publication](https://pubmed.ncbi.nlm.nih.gov/41084887/) Moxi V
### Characterization of the Intact Proteomic Profile of Senescent-Associated Secretory Phenotype by Top-Down Mass Spectrometry

The authors detached IMR-90 fibroblasts with trypsin and used a Moxi V cell analyzer to count the cells, with no further stated use of the Moxi readout for secretome normalization in the proteomics workflow.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41084887/) [Publication](https://pubmed.ncbi.nlm.nih.gov/41436442/) Moxi GO II
### Maternal acute SARS-CoV-2 infection impairs preimplantation embryo development and reprograms the early offspring hematopoietic system

The authors used the Moxi GO II to measure cell concentration and ensure optimal loading density when preparing neonatal umbilical cord blood single-cell suspensions for 10× Genomics Chromium scRNA-seq.cytometer (Orflo Technologies) to ensure optimal loading density.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/41436442/) [Publication](https://pubmed.ncbi.nlm.nih.gov/39983725/) Moxi GO II
### EZH1/EZH2 inhibition enhances adoptive T cell immunotherapy against multiple cancer models

The authors used a Moxi GO II to count engineered T-cell cultures every other day and monitor cell size/growth kinetics as a practical criterion for when the cells had rested from stimulation and were ready to proceed into downstream adoptive T-cell experiments.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/39983725/) [Publication](https://pubmed.ncbi.nlm.nih.gov/39217408/) Moxi GO II
### Attenuation of HIV-specific T cell responses Among people with HIV on ART following dipyridamole treatment

The authors used a Moxi Go II to verify post-thaw PBMC viability and accurately count cells so they could plate a consistent 1×10⁶ cells per condition for HIV Gag/Env peptide stimulation prior to intracellular cytokine staining and polyfunctionality analysis by flow cytometry.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/39217408/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40542680/) Moxi GO II
### Nectin-4 Tumor Expression as a Prognostic Factor and Potential Therapeutic Target in Stage II/III Rectal Cancer

The authors used the Moxi GO II to measure PE-labeled cell-surface Nectin-4 expression by flow cytometry in a rectal cancer cell line, providing protein-level confirmation of Nectin-4 knockdown used to interpret downstream proliferation/5-FU response assays.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40542680/) [Publication](https://pubmed.ncbi.nlm.nih.gov/39724972/) Moxi Z
### Early-life microRNA signatures in cord blood associated with allergic rhinitis and asthma development

In this study, the authors used a Moxi Z to count human airway smooth muscle cells 72 hours after miRNA mimic transfection, enabling them to quantify relative proliferation vs scramble control as functional validation for asthma- and rhinitis-associated cord-blood miRNA signatures.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/39724972/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40015107/) Moxi Z
### Major membrane protein of Mycobacterium avium subp. paratuberculosis activates immune and autophagic pathways in bovine monocyte-derived macrophages

The authors used a Moxi Z to count CD14+ bovine monocytes after magnetic enrichment and standardize seeding concentration prior to ex vivo differentiation and downstream antigen-stimulation readouts (MAPK ELISA and RNA-seq).
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40015107/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40734522/) Moxi Z
### Comparative Analysis of Inflammatory Response in Surgical Wound Drainage Fluid in Scoliosis Surgery: A Study of Neuromuscular vs. Idiopathic Patients

In this scoliosis wound-healing study, the authors used a Moxi Z to count cells recovered from POD1 surgical wound drainage after RBC lysis, then used that counted cell suspension as the input for multicolor flow-cytometry immunophenotyping to compare wound-associated leukocyte populations between idiopathic and neuromuscular patient groups.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40734522/) [Publication](https://www.sciencedirect.com/science/article/pii/S1756464625000143) Moxi GO II
### Resveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways

In this study, the authors used the Moxi Go II to quantify lysosomal activation in Lysosomal-METRIQ reporter dermal fibroblasts by measuring GFP and RFP fluorescence and computing a GREEN/RED ratio after resveratrol (and control drug) treatments, providing functional evidence that resveratrol enhances lysosome-dependent degradation pathways.
[View Publication](https://www.sciencedirect.com/science/article/pii/S1756464625000143) [Publication](https://pubmed.ncbi.nlm.nih.gov/40987405/) Moxi Z
### Butyrolactone I from Aspergillus fungi blocks neutrophil FPR1 to alleviate acute respiratory distress syndrome

The authors used a Moxi cell counter to quantify neutrophil chemotaxis by counting the number of cells that migrated through a 3-µm Boyden-chamber membrane into the lower wells after 90 minutes of fMLF-driven migration (with or without BLI pretreatment).
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40987405/) [Publication](https://doi.org/10.64898/2026.01.16.699996) Moxi GO II
### Seasonal blood-brain barrier plasticity links environmental cues to migratory behavior in monarch butterflies

The authors used a Moxi GO II to measure viability and concentration of dissociated monarch brain single-cell suspensions immediately before 10X Genomics single-cell capture for scRNA-seq.
[View Publication](https://doi.org/10.64898/2026.01.16.699996) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-PBMC-Applications.pdf) Moxi GO II Moxi V Moxi Z
### PBMC Counting + Sizing

Much of the hassle of
analyzing PBMC samples comes from the inadequate counting of the cells of interest, usually because
of debris or lack of robust counting. By circumventing imaging and going back to gold-standard
physics-based principles, every Moxi instrument gives unparalleled abilities in getting the best
cell count. With the added opportunities for fluorescence-based detection with a Moxi V and Moxi GO
II, PBMC viability checks have never been more accurate. Ensure your precious samples aren’t wasted
by making sure you get the quality checks right the first time by relying on a Moxi instrument.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-PBMC-Applications.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Compendium.pdf) Moxi GO II Moxi V Moxi Z
### Applications Compendium

Scientists are concerned with speed,
accuracy, and convenience, and those running the lab and industries are concerned with the cost
typically associated with high-performing instruments. Our proprietary Coulter Principle-based
system delivers on all three accounts, and is therefore a perfect fit for any cell biology benchtop.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Compendium.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Note-Unlocking-the-Next-Stage-in-CAR-T-Cellular-Analysis.pdf) Moxi GO II
### Unlocking the Next Stage in CAR-T Cellular Analysis

There are a variety of ways to monitor this process in cell culture, the two most prominent being
cell counting via imaging-based and coulter-based cell counting methods and higher-level cellular
analysis with fluorophore-tagged antibodies and fluorescent reporters via flow cytometry. While each
instrument used to achieve these two things has its advantages and disadvantages, few and far
between can do both at once. Out of all your options, no other instrument has the power to do both
with the speed, precision, and ease that Precision Cell Systems' Moxi Go II can. In this document,
we will take you through how you can unlock the next stage in your cellular analysis process for
CAR-T with our instruments.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Applications-Note-Unlocking-the-Next-Stage-in-CAR-T-Cellular-Analysis.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Single-Cell-Nuclei.pdf) Moxi GO II Moxi V Moxi Z
### Single Cell Nuclei Counting

Protocols for single-cell sequencing library preparation present several challenges along the way
that can impact the accuracy and quality of downstream sequencing results. Making sure you overcome
these is essential for not wasting valuable sample and money on failed sequencing runs. Precision
Cell Systems’ Moxi line of Coulter Principle-based cell analyzers enable you to address all of these
variables in a single instrument, making it the ideal go-to for all of your sequencing needs.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Single-Cell-Nuclei.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Early-Stage-Apoptosis-Monitoring-with-Annexin-V-and-PI.pdf) Moxi GO II
### Early Stage Apoptosis Monitoring

Here, we present the Precision Cell Systems Moxi GO II system as a simple, rapid, and
effective flow cytometric approach to the study of apoptosis using Annexin V
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Early-Stage-Apoptosis-Monitoring-with-Annexin-V-and-PI.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Dual-Stain-Cell-Viability-Assessment.pdf) Moxi GO II Moxi V
### Dual Stain Cell Viability Assessment

The triple-layered cell health assessment where the first layer of isolating cells from debris via
event size, the second layer of PI for staining dead cells, and the third layer of AO for all cells
or calcein for only live cells ensures that every event that is a real cell has been classified as
alive or dead and none are left uncounted. The less layers that are used, the more unreliable the
results. Multiple cell counters on the market are capable of doing this, but the accuracy and
repeatability of data generated by a Moxi Go II makes it the clear winner.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Moxi-Application-Note-Dual-Stain-Cell-Viability-Assessment.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Cell-Prep-and-Sequencing-Skin-and-Kidney-Cells-with-Parse-Evercode-WT.pdf) Singulator 100 Singulator 200
### Cell Prep and Sequencing Skin and Kidney Cells with Parse Evercode WT

The Singulator platform paired with Parse Biosciences technology enables efficient single-cell
isolation and sequencing from challenging tissue types, with high viability and robust cell
yields suitable for downstream scRNA-seq analysis.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Cell-Prep-and-Sequencing-Skin-and-Kidney-Cells-with-Parse-Evercode-WT.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Cell-Sequencing-of-Tumor-Infiltrating-Lymphocytes-in-Lung-Cancer-with-10x.pdf) Singulator 100 Singulator 200
### Single-Cell Sequencing of Tumor-Infiltrating Lymphocytes in Lung Cancer with 10x

These data demonstrate the ability of the Singulator Platform to prepare single-cell suspensions for
both positive selection by a cell surface marker and single-cell RNA sequencing. These methods
enable researchers to identify genes of interest for potential therapeutic targets for anti-tumor
response
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Cell-Sequencing-of-Tumor-Infiltrating-Lymphocytes-in-Lung-Cancer-with-10x.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Nuclei-Sequencing-of-Mouse-Cerebellum.pdf) Singulator 100 Singulator 200
### Single-Nuclei Sequencing of Mouse Cerebellum with 10x

The Singulator™ Platform provides a reproducible and precise method for isolating nuclei from
complex tissues suitable for single-nuclei RNA sequencing. Our results demonstrate consistent nuclei
yield and high-quality sequencing metrics across biological replicates, making it a reliable tool
for neuroscientific research. The ability to produce high-quality nuclei with minimal variability
enhances the accuracy of downstream snRNA analyses, facilitating deeper insights into normal biology
and disease. Finally, the ability to identify rare cell types underscores the efficacy of the
Singulator Platform in comprehensive cellular analysis, providing a detailed representation of the
cellular landscape.
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/03/Singulator-Application-Note-Single-Nuclei-Sequencing-of-Mouse-Cerebellum.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Singulator-Use-of-Nuclei-Debris-Removal-Reagent.pdf) Singulator 100 Singulator 200
### Singulator Use of Nuclei Debris Removal Reagent

The presence of intracellular and extracellular debris in single-nucleus RNA sequencing can
present a significant challenge in obtaining reliable and accurate results. However, the use of the Precision
Cell Systems Nuclei Debris Removal Reagent can effectively improve the quality of the samples. Our evaluation
of the Nuclei Debris Removal Reagent shows successful removal of debris from traditionally high-debris samples
of brain and liver nuclei while delivering high quality single nuclei suspensions. The resulting samples are
able to be run through microfluidic single nuclei sequencing platforms without issues of clogging or reduced
data quality
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2026/02/Singulator-Application-Note-Singulator-Use-of-Nuclei-Debris-Removal-Reagent.pdf) [Field Guide](/resources/02-ai-segmentation-failures/)
### AI Segmentation Failures

AI segmentation algorithms fail when encountering debris, clusters, or samples they were not trained on—resulting in minimum 3-4% error per image even under.
[Read Field Guide](/resources/02-ai-segmentation-failures/) [Blog Part 1](/resources/the-invisible-menu-part-1/)
### The Invisible Menu - Part 1: What's Really in Your Sample?

A cell count without debris quantification is like a menu without an ingredient list. Without knowing what invisible contaminants are present, every downstream decision becomes a gamble.
[Read Blog](/resources/the-invisible-menu-part-1/) [Blog Part 2](/resources/the-invisible-menu-part-2/)
### The Invisible Menu - Part 2: The Five Courses You Didn't Order

Five contaminants corrupt every sample. Image counters exclude them from counts but never reveal their presence. Until you can quantify what's actually in your sample—not just how many cells—these villains control the menu.
[Read Blog](/resources/the-invisible-menu-part-2/) [Blog Part 3](/resources/the-invisible-menu-part-3-physics-vs-pixels/)
### The Invisible Menu - Part 3: Physics vs. Pixels

Better AI won't save your data. More training images won't expose the contaminants. Faster cameras won't quantify your debris. Physics will. The Coulter principle—the same physics that transformed clinical hematology—offers research laboratories what imaging never can: direct measurement of what's actually in your sample.
[Read Blog](/resources/the-invisible-menu-part-3-physics-vs-pixels/) [Blog Part 4](/resources/the-invisible-menu-part-4/)
### The Invisible Menu - Part 4: The Recipe Revealed

The recipe for defeating the five villains isn't better algorithms or faster cameras. It's physics-based measurement that reveals what image counters hide: the complete composition of your sample. Direct size measurement. Complete population visualization. Standardized thresholds. Informed decisions. That's the recipe.
[Read Blog](/resources/the-invisible-menu-part-4/) [Blog Part 5](/resources/the-invisible-menu-part-5/)
### The Invisible Menu - Part 5: The Clean Kitchen

Every laboratory has an invisible menu. Hidden ingredients contaminate samples. Villains corrupt data. Resources get wasted on samples that should have been cleaned up first. The question isn't whether these problems exist—they do, in every laboratory that relies on image-based counting alone. The question is whether you'll continue ordering blind, or finally demand to read the full ingredient list. Physics-based debris quantification isn't just an alternative to image counting. It's the missing QC checkpoint that transforms sample preparation from guesswork to measurement. From hope to confidence. From invisible menus to clean kitchens. The recipe is proven. The villains are defeated. The kitchen can be clean.
[Read Blog](/resources/the-invisible-menu-part-5/) [Publication](https://pubmed.ncbi.nlm.nih.gov/40236205/) Moxi Z
### CHD7 binds distinct regions in the Sox11 locus to regulate neuronal differentiation

In this study, the authors used Moxi to standardize plated cell numbers and per-reaction cell inputs for proliferation, neuronal differentiation phenotyping, and genome-wide CHD7 occupancy assays in iMOP progenitors and iMOP-derived neurons.
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40236205/) [Field Guide](/resources/07-single-cell-loading-decision/)
### Single-Cell Loading Decision Framework: Confident Go/No-Go Before Chip Commitment

Every single-cell experiment represents a significant investment—consumables alone cost $500–1000+ per chip, plus downstream sequencing—so a confident go/no-go decision before chip commitment protects the run.
[Read Field Guide](/resources/07-single-cell-loading-decision/) [Blog Part 1](/resources/unlocking-ffpe-archives-part-1-the-ffpe-fortune/) Singulator 200+
### Unlocking FFPE Archives - Part 1: The FFPE Fortune - What's Hidden in Your Biobank?

Somewhere in your institution, there's a dusty shelf. Sitting on it: thousands of tissue blocks. Each one holds decades of clinical history. Patient outcomes. Treatment responses. Disease progression. Data that took years to collect. And can you blame researchers for walking past it?
[Read Blog](/resources/unlocking-ffpe-archives-part-1-the-ffpe-fortune/) [Blog Part 2](/resources/unlocking-ffpe-archives-part-2-ffpe-chemistry-problem-preservation-prison/) Singulator 200+
### Unlocking FFPE Archives - Part 2: The Chemistry Problem: Why Preservation Became a Prison

Here's a weird fact: the same chemical that saves your sample also traps everything useful inside it. Think about laminating a document. Great for protection. Terrible if you need to edit what's inside. That's formalin. And for decades, nobody could undo the damage.
[Read Blog](/resources/unlocking-ffpe-archives-part-2-ffpe-chemistry-problem-preservation-prison/) [Blog Part 3](/resources/unlocking-ffpe-archives-part-3-manual-vs-modern-workflow-debate/) Singulator 200+
### Unlocking FFPE Archives - Part 3: Manual Methods vs. Modern Science: The Great Workflow Debate

Third try. Third failure. The fume hood is running. The xylene smells terrible. And the protocol that worked last Tuesday is giving nothing but debris. Sound familiar? Here's the question nobody was asking: Is it your technique—or is it the physics?
[Read Blog](/resources/unlocking-ffpe-archives-part-3-manual-vs-modern-workflow-debate/) [Blog Part 4](/resources/unlocking-ffpe-archives-part-4-two-hours-sixty-minutes-automation/) Singulator 200+
### Unlocking FFPE Archives - Part 4: From Two Hours to Sixty Minutes: What Automation Actually Changes

What if that two-hour protocol took sixty minutes? What if twelve pipetting steps dropped to four? What if the fume hood became optional? These aren't hypotheticals. This is what purpose-built automation looks like. And the difference isn't just time.
[Read Blog](/resources/unlocking-ffpe-archives-part-4-two-hours-sixty-minutes-automation/) [Blog Part 5](/resources/unlocking-ffpe-archives-part-5-the-archive-opens-are-you-ready/) Singulator 200+
### Unlocking FFPE Archives - Part 5: The Archive Opens: Are You Ready?

Behind every tissue block is a patient who said yes. Yes to collection. Yes to research. Yes to the hope that their gift might help someone else. They trusted the science. The question now: are the protocols worthy of that trust?
[Read Blog](/resources/unlocking-ffpe-archives-part-5-the-archive-opens-are-you-ready/) [Publication](https://www.biorxiv.org/content/10.1101/2025.03.13.643011v2) Singulator 100
### Spatial Transcriptomics and Single-Nucleus RNA Sequencing Reveal rAAV2- and rAAV9-Specific Transduction Signatures in the Mouse Liver
[View Publication](https://www.biorxiv.org/content/10.1101/2025.03.13.643011v2) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12345466/) Singulator 100
### Single-Nucleus RNA Sequencing and Spatial Transcriptomics Reveal Cellular Heterogeneity and Intercellular Communication Networks in the Hypothalamus-Pituitary-Ovarian Axis of Pregnant Mongolian Cattle
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12345466/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12259082/) Singulator 200+
### Oncogenic and tumor-suppressive forces converge on a progenitor-orchestrated niche to shape early tumorigenesis
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12259082/) [Publication](https://www.nature.com/articles/s44161-025-00698-y) Singulator 100
### Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis
[View Publication](https://www.nature.com/articles/s44161-025-00698-y) [Publication](https://www.biorxiv.org/content/10.1101/2025.06.25.661527v1) Singulator 100
### A porcine germ cell depletion model to investigate the role of germ cells in gonadal development
[View Publication](https://www.biorxiv.org/content/10.1101/2025.06.25.661527v1) [Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/S200_Brochure_final_v1.pdf) Singulator 200+
### Brochure - Singulator 200+
[Download Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/S200_Brochure_final_v1.pdf) [Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/S200_Brochure_FFPE_final_v1.pdf) Singulator 200+
### Brochure - S200+ - FFPE
[Download Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/S200_Brochure_FFPE_final_v1.pdf) [Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/Moxi_GO_II_Brochure.pdf) Moxi GO II
### Brochure - Moxi GO II
[Download Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/Moxi_GO_II_Brochure.pdf) [Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/Moxi-Z_V-Brochure.pdf) Moxi V Moxi Z
### Brochure - Moxi V + Z
[Download Brochure](https://precisioncellsystems.com/wp-content/uploads/2025/12/Moxi-Z_V-Brochure.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/11/Applicaiton_Note_Singulator_FFPE_v1_11-30-25.pdf) Singulator 200+
### FFPE Tissue Dissociation
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/11/Applicaiton_Note_Singulator_FFPE_v1_11-30-25.pdf) [Blog Part 3](/resources/brain-map-part-3-fresh-vs-frozen-which-side-are-you-on/) Singulator 100 Singulator 200 Singulator 200+
### Brain Map - Part 3: Fresh vs. Frozen: Which Side Are You On?

Fresh vs. Frozen: Which Side Are You On? Description: The debate between fresh tissue (whole cells) and frozen tissue (nuclei) divides labs. We explore why fresh dissociation often creates a "map of a disaster" through stress artifacts, and why frozen nuclei offer the unbiased, stable truth required for atlas-scale science.
[Read Blog](/resources/brain-map-part-3-fresh-vs-frozen-which-side-are-you-on/) [Blog Part 4](/resources/brain-map-part-4-the-list-is-good-but-is-the-map-better/) Singulator 100 Singulator 200 Singulator 200+
### Brain Map - Part 4: The List is Good, But Is the Map Better?

The List is Good, But Is the Map Better? Description: Single-nucleus sequencing gives you the "List" of cell types, but Spatial Transcriptomics gives you the "Map." Discover how combining these technologies creates a high-resolution "Precision Point," and how one automated platform can serve as the engine for both workflows.
[Read Blog](/resources/brain-map-part-4-the-list-is-good-but-is-the-map-better/) [Blog Part 5](/resources/brain-map-part-5-the-journey-ends-here-are-you-ready-to-build-the-map/) Singulator 100 Singulator 200 Singulator 200+
### Brain Map - Part 5: The Journey Ends Here - Are You Ready to Build the Map?

From the hidden world of glia to the final 3D atlas, the journey of modern neuroscience relies on one foundational step: sample preparation. We conclude our series by challenging researchers to prioritize clean, reproducible input to build the definitive map of the human brain.
[Read Blog](/resources/brain-map-part-5-the-journey-ends-here-are-you-ready-to-build-the-map/) [Blog Part 1](/resources/brain-map-part-1-why-did-science-miss-half-the-brain/) Singulator 100 Singulator 200 Singulator 200+
### Brain Map - Part 1: Why Did Science Miss Half the Brain?

For a century, neuroscience focused exclusively on the neuron, dismissing glia as mere "packing peanuts." Discover how single-nucleus sequencing revealed the active, critical role of the brain's immune and support systems—and why this shift changes everything for Alzheimer's research.
[Read Blog](/resources/brain-map-part-1-why-did-science-miss-half-the-brain/) [Blog Part 2](/resources/brain-map-part-2-what-is-ruining-your-frozen-experiments/) Singulator 100 Singulator 200 Singulator 200+
### Brain Map - Part 2: What is Ruining Your Frozen Experiments?

What is Ruining Your Frozen Experiments? Description: Every great story needs a villain. In frozen brain tissue processing, that villain is myelin debris. Learn how lipid contamination clogs microfluidics and ruins data, and see how the Singulator’s automated Protocol DP0006 neutralizes this threat to unlock biobank archives.
[Read Blog](/resources/brain-map-part-2-what-is-ruining-your-frozen-experiments/) [Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/11/2025-11-05_Precision-Cell-Systems_-Automating-Tissue-Dissociation-for-Single-Cell-Analysis.mp4) Singulator 100 Singulator 200
### Webinar 11-5-25: Automating Tissue Dissociation for Single Cell Analysis
[Watch Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/11/2025-11-05_Precision-Cell-Systems_-Automating-Tissue-Dissociation-for-Single-Cell-Analysis.mp4) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC9485887/) Moxi Z
### The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC9485887/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5570934/) Moxi Z
### Smac mimetics and oncolytic viruses synergize in driving anticancer T-cell responses through complementary mechanisms
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5570934/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12061129/) Moxi GO II
### Small-scale protocols to characterize mitochondrial Complex V activity and assembly in peripheral blood mononuclear cells
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12061129/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7842692/) Moxi Z
### Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7842692/) [Publication](https://www.cell.com/iscience/fulltext/S2589-0042(25)01158-7) Moxi GO II
### Simulating CD8 T cell exhaustion: A comprehensive approach
[View Publication](https://www.cell.com/iscience/fulltext/S2589-0042(25)01158-7) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12339179/) Moxi GO II
### Secretion of specific metabolites and changes in miRNA expression in murine osteoblastic cells exposed in vitro to α-radiation
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12339179/) [Publication](https://foliabiologica.lf1.cuni.cz/media/pdf/fb_2019065020064.pdf) Moxi GO II
### The CD34 + Cell Number Alone Predicts Retention of theHuman Fat-Graft Volume in a Nude Mouse Model
[View Publication](https://foliabiologica.lf1.cuni.cz/media/pdf/fb_2019065020064.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/Research-Spotlight-Optimizing-Nuclei-Isolation-for-Reliable-snRNA-seq-A-Comparative-Study-Validates-the-SingulatorTM-Platform.pdf) Singulator 100 Singulator 200
### Optimizing Nuclei Isolation for Reliable snRNA-seq - A Comparative Study Validates the Singulator Platform
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/Research-Spotlight-Optimizing-Nuclei-Isolation-for-Reliable-snRNA-seq-A-Comparative-Study-Validates-the-SingulatorTM-Platform.pdf) [Publication](https://pubmed.ncbi.nlm.nih.gov/40504625/) Moxi GO II
### Device-free isolation of photoreceptor cells from patient iPSC-derived retinal organoids
[View Publication](https://pubmed.ncbi.nlm.nih.gov/40504625/) [Publication](https://pubmed.ncbi.nlm.nih.gov/30283141/) Moxi GO II
### Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
[View Publication](https://pubmed.ncbi.nlm.nih.gov/30283141/) [Publication](https://www.nature.com/articles/s42003-020-01625-6) Moxi GO II
### Mapping the immune landscape of clear cell renal cell carcinoma by single-cell RNA-seq
[View Publication](https://www.nature.com/articles/s42003-020-01625-6) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Single-Cell-Tech-Tip.pdf) Moxi GO II Moxi V Moxi Z
### Moxi Single Cell Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Single-Cell-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Single-or-Dual-Color-Viability-Staining-Tech-Tip.pdf) Moxi GO II Moxi V
### Moxi Single or Dual Color Viability Staining Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Single-or-Dual-Color-Viability-Staining-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-21-CFR-Part-11-Tech-Tip.pdf) Moxi GO II Moxi V
### Moxi 21 CFR Part 11 Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-21-CFR-Part-11-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-CAR-T-Tech-Tip.pdf) Moxi GO II
### Moxi CAR-T Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-CAR-T-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Consumables-Tech-Tip.pdf) Moxi GO II Moxi V Moxi Z
### Moxi Consumables Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Consumables-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Fluorescence-Based-Reporter-Checks-Tech-Tip.pdf) Moxi GO II
### Moxi Fluorescence-Based Reporter Checks Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Fluorescence-Based-Reporter-Checks-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Immunoprofiling-Tech-Tip.pdf) Moxi GO II
### Moxi Immunoprofiling Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Immunoprofiling-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Immunotherapy-Tech-Tip.pdf) Moxi GO II
### Moxi Immunotherapy Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Immunotherapy-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-IQOQ-Validation-Tech-Tip.pdf) Moxi GO II Moxi V
### Moxi IQOQ Validation Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-IQOQ-Validation-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Moxi-System-Validation-Tech-Tip.pdf) Moxi GO II Moxi V Moxi Z
### Moxi System Validation Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Moxi-System-Validation-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Moxi-Systems-Coulter-vs-Image-Based-Counting-Tech-Tip.pdf) Moxi GO II Moxi V Moxi Z
### Moxi Systems Coulter vs Image Based Counting Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Moxi-Systems-Coulter-vs-Image-Based-Counting-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Nuclei-Counting-Tech-Tip.pdf) Moxi GO II Moxi V
### Moxi Nuclei Counting Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-Nuclei-Counting-Tech-Tip.pdf) [Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-PBMC-Counting-and-Viability-Tech-Tip.pdf) Moxi GO II Moxi V Moxi Z
### Moxi PBMC Counting and Viability Tech Note
[Read Tech Note](https://precisioncellsystems.com/wp-content/uploads/2025/09/ORFLO-PBMC-Counting-and-Viability-Tech-Tip.pdf) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11259153/) Moxi GO II
### Streamlined measurement of chimeric antigen receptor T-cell concentration, size, viability and two-color phenotyping during manufacturing
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11259153/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11631952/) Moxi Z
### Organoclay flocculation as a pathway to export carbon from the sea surface
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11631952/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11815381/) Moxi GO II
### Tumor-treating fields and concurrent cisplatin chemotherapy display synergistic effects on triple-negative breast cancer cells
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11815381/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11917452/) Moxi GO II
### CD5 deletion enhances the antitumor activity of adoptive T cell therapies
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11917452/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC4850312/) Moxi Z
### Strain-specific Loss of Formyl Peptide Receptor 3 in the Murine Vomeronasal and Immune Systems
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC4850312/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5083104/) Moxi Z
### Contribution of Human Fibroblasts and Endothelial Cells to the Hallmarks of Inflammation as Determined by Proteome Profiling
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5083104/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5088566/) Moxi Z
### Condensin II mutation causes T-cell lymphoma through tissue-specific genome instability
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5088566/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5170857/) Moxi GO II
### Roles for E-cadherin cell surface regulation in cancer
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5170857/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5350271/) Moxi Z
### Neurons derived from different brain regions are inherently different in vitro: a novel multiregional brain-on-a-chip
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5350271/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5511375/) Moxi GO II
### A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5511375/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5546990/) Moxi Z
### Asthma remission: Predicting future airways responsiveness using an miRNA network
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5546990/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5622101/) Moxi Z
### Methylene blue inhibits NLRP3, NLRC4, AIM2, and non-canonical inflammasome activation
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5622101/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5666678/) Moxi Z
### Integrator orchestrates RAS/ERK1/2 signaling transcriptional programs
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5666678/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5795392/) Moxi Z
### Proteomics and metabolomics identify molecular mechanisms of aging potentially predisposing for chronic lymphocytic leukemia
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5795392/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5828198/) Moxi Z
### The relationship of NM23 (NME) metastasis suppressor histidine phosphorylation to its nucleoside diphosphate kinase, histidine protein kinase and motility functions
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5828198/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5918346/) Moxi GO II
### High-yield purification, preservation, and serial transplantation of human satellite cells
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5918346/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5944590/) Moxi GO II
### Prolonged cenicriviroc therapy reduces hepatic fibrosis despite steatohepatitis in a diet-induced mouse model of nonalcoholic steatohepatitis
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5944590/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5945276/) Moxi Z
### Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC5945276/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6054483/) Moxi Z
### Serine availability influences mitochondrial dynamics and function through lipid metabolism
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6054483/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6107272/) Moxi Z
### Pulmonary influenza A virus infection leads to suppression of the innate immune response to dermal injury
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6107272/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6167529/) Moxi GO II
### PET of adoptively transferred chimeric antigen receptor T cells with 89Zr-oxine
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6167529/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6171522/) Moxi Z
### Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6171522/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6173642/) Moxi Z
### Acetate production from glucose and coupling to mitochondrial metabolism in mammalsDe novo acetate production is coupled to central carbon metabolism in mammals
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6173642/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6217722/) Moxi Z
### Coinfection with influenza A virus and Klebsiella oxytoca: an underrecognized impact on host resistance and tolerance to pulmonary infections
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6217722/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6303315/) Moxi Z
### Serine synthesis through PHGDH coordinates nucleotide levels by maintaining central carbon metabolism
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6303315/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6584668/) Moxi Z
### Single and fractionated ionizing radiation induce alterations in endothelial connexin expression and channel function
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6584668/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6713228/) Moxi Z
### Bespoke pretargeted nanoradioimmunotherapy for the treatment of non-Hodgkin lymphoma
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6713228/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6984401/) Moxi GO II
### Generation of an immortalized erythroid progenitor cell line from peripheral blood: A model system for the functional analysis of Plasmodium spp. invasion
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6984401/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6335469/) Moxi Z
### The CHD6 chromatin remodeler is an oxidative DNA damage response factor
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6335469/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7461615/) Moxi GO II
### Paneth cell-derived lysozyme defines the composition of mucolytic microbiota and the inflammatory tone of the intestine
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7461615/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7504913/) Moxi GO II
### Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7504913/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8324880/) Moxi GO II
### Sirtuin 5 depletion impairs mitochondrial function in human proximal tubular epithelial cells
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8324880/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11789634/) Moxi GO II
### NKX2.2 and KLF4 cooperate to regulate α-cell identity
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11789634/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC9298545/) Moxi Z
### Pyruvate Supports RET-Dependent Mitochondrial ROS Production to Control Mycobacterium avium Infection in Human Primary Macrophages
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC9298545/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC9036621/) Moxi Z
### Naegleria's mitotic spindles are built from unique tubulins and highlight core spindle features
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC9036621/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8764040/) Moxi Z
### Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8764040/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8981723/) Moxi Z
### Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell Anergy
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8981723/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8584748/) Moxi GO II
### The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8584748/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7946769/) Moxi Z
### Functional analysis of immune signature genes in Th1* memory cells links ISOC1 and pyrimidine metabolism to IFNγ and IL-17 production
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7946769/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8222825/) Moxi GO II
### Endothelial Aryl Hydrocarbon Receptor Nuclear Translocator Mediates the Angiogenic Response to Peripheral Ischemia in Mice With Type 2 Diabetes Mellitus
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8222825/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8511570/) Moxi GO II
### Lateral lumbar interbody fusion using a cellular allogeneic bone matrix in the treatment of symptomatic degenerative lumbar disc disease and lumbar spinal stenosis
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8511570/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7958435/) Moxi Z
### Monocyte markers correlate with immune and neuronal brain changes in REM sleep behavior disorder
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7958435/) [Publication](https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30877-7?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1534580720308777%3Fshowall%3Dtrue) Moxi GO II
### A Single-Cell Transcriptomic Atlas of Human Skin Aging
[View Publication](https://www.cell.com/developmental-cell/fulltext/S1534-5807(20)30877-7?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1534580720308777%3Fshowall%3Dtrue) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7063048/) Moxi GO II
### Mitochondria as target to inhibit proliferation and induce apoptosis of cancer cells: The effects of doxycycline and gemcitabine
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7063048/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6996358/) Moxi Z
### The snoRNA target of t (4; 14) in multiple myeloma regulates ribosome biogenesis
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6996358/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7066501/) Moxi Z
### Connexin43 hemichannel targeting with TAT-Gap19 alleviates radiation-induced endothelial cell damage
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7066501/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6390935/) Moxi Z
### Evidence for an alternative fatty acid desaturation pathway increasing cancer plasticity
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6390935/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6722131/) Moxi GO II
### High-throughput-compatible assays using a genetically-encoded calcium indicator
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6722131/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6477081/) Moxi Z
### Canagliflozin inhibits human endothelial cell proliferation and tube formation
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6477081/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6532103/) Moxi GO II
### Viral Factors Important for Efficient Replication of Influenza A Viruses in Cells of the Central Nervous System
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC6532103/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8240505/) Moxi GO II
### A Single Cell Transcriptomic Atlas Characterizes Aging Tissues in the Mouse
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC8240505/) [Publication](https://pubs.rsc.org/en/content/articlelanding/2018/lc/c7lc01005b) Moxi Z
### Microfluidic label-free selection of mesenchymal stem cell subpopulation during culture expansion extends the chondrogenic potential in vitro
[View Publication](https://pubs.rsc.org/en/content/articlelanding/2018/lc/c7lc01005b) [Publication](https://www.cell.com/cell-reports/fulltext/S2211-1247(16)31281-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2211124716312815%3Fshowall%3Dtrue) Moxi Z
### Breast cancer-derived lung metastases show increased pyruvate carboxylase-dependent anaplerosis
[View Publication](https://www.cell.com/cell-reports/fulltext/S2211-1247(16)31281-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2211124716312815%3Fshowall%3Dtrue) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11108266/) Moxi Z
### Efficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11108266/) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11974938/) Singulator 100 Singulator 200
### Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC11974938/) [Publication](https://www.biorxiv.org/content/10.1101/2024.11.12.623261v1) Moxi GO II
### Human skin rejuvenation via mRNA
[View Publication](https://www.biorxiv.org/content/10.1101/2024.11.12.623261v1) [Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/08/Singulator-Session-III.mp4) Singulator 100 Singulator 200
### Singulator Sessions III: Unlock the Potential of Your Neuroscience Samples with the Singulator Platform Singulator Session III
[Watch Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/08/Singulator-Session-III.mp4) [Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/08/Singulator-Session-II-Oncology-Applications.mp4) Singulator 100 Singulator 200
### Singulator Sessions II: Leveraging the Singulator Platform for Oncology Applications
[Watch Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/08/Singulator-Session-II-Oncology-Applications.mp4) [Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/08/Singulator-Session-I.mp4) Singulator 100 Singulator 200
### Singulator Session I: Automating Tissue Dissociation for Single-Cell Analysis
[Watch Webinar](https://precisioncellsystems.com/wp-content/uploads/2025/08/Singulator-Session-I.mp4) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Average-Cell-Size-By-Cell-Type.pdf) Moxi GO II Moxi V Moxi Z
### Moxi - Average Cell Size By Cell Type
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Average-Cell-Size-By-Cell-Type.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Car-T-Activation-Monitoring.pdf) Moxi GO II
### Moxi - CAR-T Activation Monitoring
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Car-T-Activation-Monitoring.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Immunophenotyping-Applications.pdf) Moxi GO II
### Moxi - Immunophenotyping Applications
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Immunophenotyping-Applications.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Monitoring-Cell-Culture-Health-and-MPI-With-the-Moxi-Z.pdf) Moxi Z
### Moxi - Monitoring Cell Culture Health and MPI With the Moxi Z
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Monitoring-Cell-Culture-Health-and-MPI-With-the-Moxi-Z.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Yeast-and-Craft-Beer-Applications.pdf) Moxi GO II Moxi V Moxi Z
### Moxi - Yeast and Craft Beer Applications
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Yeast-and-Craft-Beer-Applications.pdf) [App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Algae-Cell-Counting.pdf) Moxi GO II Moxi V Moxi Z
### Moxi - Algae Cell Counting
[Download App Note](https://precisioncellsystems.com/wp-content/uploads/2025/08/Moxi-Algae-Cell-Counting.pdf) [Publication](https://precisioncellsystems.com/wp-content/uploads/2025/10/s41586-022-05450-1.pdf) Singulator 100 Singulator 200
### Kinase signaling in excitatory neurons regulates sleep quantity and depth
[View Publication](https://precisioncellsystems.com/wp-content/uploads/2025/10/s41586-022-05450-1.pdf) [Publication](https://www.biorxiv.org/content/10.1101/2023.09.11.557118v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.28.1743558253016&__hsfp=1300058700&submissionGuid=f991745a-6dc4-444a-8d60-f34230c118e0) Singulator 100 Singulator 200
### Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by single-cell transcriptomics
[View Publication](https://www.biorxiv.org/content/10.1101/2023.09.11.557118v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.28.1743558253016&__hsfp=1300058700&submissionGuid=f991745a-6dc4-444a-8d60-f34230c118e0) [Publication](https://www.science.org/doi/10.1126/scitranslmed.abq0476?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.30.1743558253016&__hsfp=1300058700&submissionGuid=1bb5f085-dcf1-4a34-a999-052cc6cad242) Singulator 100 Singulator 200
### Tissue-specific features of the T cell repertoire after allogeneic hematopoietic cell transplantation in human and mouse
[View Publication](https://www.science.org/doi/10.1126/scitranslmed.abq0476?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.30.1743558253016&__hsfp=1300058700&submissionGuid=1bb5f085-dcf1-4a34-a999-052cc6cad242) [Publication](https://link.springer.com/article/10.1007/s00401-023-02604-x?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.31.1743558253016&__hsfp=1300058700&submissionGuid=c7f3c611-02ec-4ec0-a180-2e24c56a4025) Singulator 100 Singulator 200
### Cell-specific MAPT gene expression is preserved in neuronal and glial tau cytopathologies in progressive supranuclear palsy
[View Publication](https://link.springer.com/article/10.1007/s00401-023-02604-x?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.31.1743558253016&__hsfp=1300058700&submissionGuid=c7f3c611-02ec-4ec0-a180-2e24c56a4025) [Publication](https://www.nature.com/articles/s41467-023-36344-z?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.33.1743558253016&__hsfp=1300058700&submissionGuid=287fece2-b893-47b1-82f8-69ed503994a0) Singulator 100 Singulator 200
### Application of high-throughput, high-depth, targeted single-nucleus DNA sequencing in pancreatic cancer
[View Publication](https://www.nature.com/articles/s41467-023-36344-z?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.33.1743558253016&__hsfp=1300058700&submissionGuid=287fece2-b893-47b1-82f8-69ed503994a0) [Publication](https://elifesciences.org/articles/81090?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.27.1743558253016&__hsfp=1300058700&submissionGuid=d621ffa4-d176-4293-828a-2ca2f3c1f2e6) Singulator 100 Singulator 200
### Single-nuclei characterization of pervasive transcriptional signatures across organs in response to COVID-19
[View Publication](https://elifesciences.org/articles/81090?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.27.1743558253016&__hsfp=1300058700&submissionGuid=d621ffa4-d176-4293-828a-2ca2f3c1f2e6) [Publication](https://www.biorxiv.org/content/10.1101/2023.11.17.567534v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.26.1743558253016&__hsfp=1300058700&submissionGuid=838128d8-35f7-458b-a0a0-6e5e93506818) Singulator 100 Singulator 200
### Sexually dimorphic role of diet and stress on behavior, energy metabolism, and the ventromedial hypothalamus
[View Publication](https://www.biorxiv.org/content/10.1101/2023.11.17.567534v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.26.1743558253016&__hsfp=1300058700&submissionGuid=838128d8-35f7-458b-a0a0-6e5e93506818) [Presentation](https://sail.mskcc.org/research/experimental-research/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743110139007.1743114390284.6&__hssc=225964101.1.1743114390284&__hsfp=1300058700&submissionGuid=da42544b-9315-47f8-87f4-f27614a8159e) Singulator 100 Singulator 200
### Comparison of polydT and probe-based RNA capture for single-cell RNAseq
[View Presentation](https://sail.mskcc.org/research/experimental-research/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743110139007.1743114390284.6&__hssc=225964101.1.1743114390284&__hsfp=1300058700&submissionGuid=da42544b-9315-47f8-87f4-f27614a8159e) [Protocol](https://www.protocols.io/view/nuclei-isolation-from-human-meniscus-for-10x-multi-eq2lyweqwvx9/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743110139007.1743114390284.6&__hssc=225964101.3.1743114390284&__hsfp=1300058700&submissionGuid=4b05fc85-7463-4780-a2b2-ffab27dfab93) Singulator 100 Singulator 200
### Nuclei Isolation from Human Meniscus for 10x Multiome
[Read Protocol](https://www.protocols.io/view/nuclei-isolation-from-human-meniscus-for-10x-multi-eq2lyweqwvx9/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743110139007.1743114390284.6&__hssc=225964101.3.1743114390284&__hsfp=1300058700&submissionGuid=4b05fc85-7463-4780-a2b2-ffab27dfab93) [Protocol](https://www.protocols.io/view/nuclei-isolation-from-human-synovium-and-infrapate-ewov193molr2/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.1.1743120525713&__hsfp=1300058700&submissionGuid=b0e5a9f5-043f-4bd6-aed3-b4f40dec0679) Singulator 100 Singulator 200
### Nuclei Isolation from Human Synovium and Infrapatellar Fat Pad for 10x Multiome
[Read Protocol](https://www.protocols.io/view/nuclei-isolation-from-human-synovium-and-infrapate-ewov193molr2/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.1.1743120525713&__hsfp=1300058700&submissionGuid=b0e5a9f5-043f-4bd6-aed3-b4f40dec0679) [Protocol](https://www.protocols.io/view/nuclei-isolation-from-human-cartilage-for-10x-mult-rm7vzj7q5lx1/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.2.1743120525713&__hsfp=1300058700&submissionGuid=de8f645d-64af-4162-9628-0577ae4400f2) Singulator 100 Singulator 200
### Nuclei Isolation from Human Cartilage for 10x Multiome
[Read Protocol](https://www.protocols.io/view/nuclei-isolation-from-human-cartilage-for-10x-mult-rm7vzj7q5lx1/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.2.1743120525713&__hsfp=1300058700&submissionGuid=de8f645d-64af-4162-9628-0577ae4400f2) [Protocol](https://www.protocols.io/view/1-perff-seq-cell-and-nuclei-preparation-c8n3zvgn.html?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.3.1743120525713&__hsfp=1300058700&submissionGuid=cb652c05-65bb-4462-94bc-f6d6270950ee) Singulator 200+
### PERFF-seq: Cell and Nuclei Preparation
[Read Protocol](https://www.protocols.io/view/1-perff-seq-cell-and-nuclei-preparation-c8n3zvgn.html?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.3.1743120525713&__hsfp=1300058700&submissionGuid=cb652c05-65bb-4462-94bc-f6d6270950ee) [Protocol](https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.848?__hsfp=1300058700&__hssc=225964101.4.1743120525713&__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&submissionGuid=51d58e6d-a114-45fd-a519-2d936a8859b0) Singulator 100 Singulator 200
### Systematic Sampling of the Female Reproductive System for Molecular Characterization
[Read Protocol](https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.848?__hsfp=1300058700&__hssc=225964101.4.1743120525713&__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&submissionGuid=51d58e6d-a114-45fd-a519-2d936a8859b0) [Protocol](https://www.jove.com/t/65611/a-simple-quick-partially-automated-protocol-for-isolation-single?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.6.1743120525713&__hsfp=1300058700&submissionGuid=d251dea3-f5b9-441b-b0eb-4eadf390de7e) Singulator 100 Singulator 200
### A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
[Read Protocol](https://www.jove.com/t/65611/a-simple-quick-partially-automated-protocol-for-isolation-single?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.6.1743120525713&__hsfp=1300058700&submissionGuid=d251dea3-f5b9-441b-b0eb-4eadf390de7e) [Protocol](https://www.protocols.io/view/nuclei-extraction-for-single-cell-rnaseq-from-froz-q26g74xzqgwz/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.13.1743120525713&__hsfp=1300058700&submissionGuid=cc1fb887-2fc0-423d-9124-6e7264b1d55f) Singulator 100 Singulator 200
### Nuclei extraction for single-cell RNAseq from frozen tissue using Singulator™ 100
[Read Protocol](https://www.protocols.io/view/nuclei-extraction-for-single-cell-rnaseq-from-froz-q26g74xzqgwz/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.13.1743120525713&__hsfp=1300058700&submissionGuid=cc1fb887-2fc0-423d-9124-6e7264b1d55f) [Protocol](https://www.protocols.io/view/tissue-dissociation-for-multiome-analysis-using-s2-yxmvmndx6g3p/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.14.1743120525713&__hsfp=1300058700&submissionGuid=8ee42fe6-6ea0-48eb-bbd2-8bdaf97d156b) Singulator 100 Singulator 200
### Tissue Dissociation for Multiome Analysis Using S2 Singulator
[Read Protocol](https://www.protocols.io/view/tissue-dissociation-for-multiome-analysis-using-s2-yxmvmndx6g3p/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.14.1743120525713&__hsfp=1300058700&submissionGuid=8ee42fe6-6ea0-48eb-bbd2-8bdaf97d156b) [Data Set](https://www.parsebiosciences.com/datasets/performance-of-evercode-wt-v3-in-mouse-lymph-node-nuclei/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.5.1743120525713&__hsfp=1300058700&submissionGuid=ccd705a7-ba96-482f-b624-df6abb8a7201) Singulator 100 Singulator 200
### Parse Biosciences: Performance of Evercode™ WT in Mouse Lymph Node
[View Data Set](https://www.parsebiosciences.com/datasets/performance-of-evercode-wt-v3-in-mouse-lymph-node-nuclei/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.5.1743120525713&__hsfp=1300058700&submissionGuid=ccd705a7-ba96-482f-b624-df6abb8a7201) [Data Set](https://www.parsebiosciences.com/datasets/lung/performance-of-evercode-wt-in-mouse-lung-nuclei/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.15.1743120525713&__hsfp=1300058700&submissionGuid=9634964b-b524-4b31-9aa1-f4d35dbada04) Singulator 100 Singulator 200
### Parse Biosciences: Performance of Evercode™ WT in Mouse Lung Nuclei
[View Data Set](https://www.parsebiosciences.com/datasets/lung/performance-of-evercode-wt-in-mouse-lung-nuclei/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.15.1743120525713&__hsfp=1300058700&submissionGuid=9634964b-b524-4b31-9aa1-f4d35dbada04) [Data Set](https://www.parsebiosciences.com/datasets/performance-of-evercode-wt-in-mouse-kidney-cells/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.16.1743120525713&__hsfp=1300058700&submissionGuid=07d1b598-d2a7-4804-bf9d-fb8f317d8d10) Singulator 100 Singulator 200
### Parse Biosciences: Performance of Evercode™ WT in Mouse Kidney Cells - 2
[View Data Set](https://www.parsebiosciences.com/datasets/performance-of-evercode-wt-in-mouse-kidney-cells/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.16.1743120525713&__hsfp=1300058700&submissionGuid=07d1b598-d2a7-4804-bf9d-fb8f317d8d10) [Data Set](https://www.parsebiosciences.com/datasets/performance-of-evercode-wt-in-mouse-kidney-nuclei/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.17.1743120525713&__hsfp=1300058700&submissionGuid=4fb6fdf7-f4f1-4da0-a5ba-a82428f174e0) Singulator 100 Singulator 200
### Parse Biosciences: Performance of Evercode™ WT in Mouse Kidney Nuclei
[View Data Set](https://www.parsebiosciences.com/datasets/performance-of-evercode-wt-in-mouse-kidney-nuclei/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.17.1743120525713&__hsfp=1300058700&submissionGuid=4fb6fdf7-f4f1-4da0-a5ba-a82428f174e0) [Data Set](https://www.parsebiosciences.com/datasets/skin/performance-of-evercode-wt-in-mouse-skin-cells/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.18.1743120525713&__hsfp=1300058700&submissionGuid=c1dab13d-ab5d-4618-b08c-39d416e1c057) Singulator 100 Singulator 200
### Parse Biosciences: Performance of Evercode™ WT in Mouse Skin Cells
[View Data Set](https://www.parsebiosciences.com/datasets/skin/performance-of-evercode-wt-in-mouse-skin-cells/?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743114390284.1743120525713.7&__hssc=225964101.18.1743120525713&__hsfp=1300058700&submissionGuid=c1dab13d-ab5d-4618-b08c-39d416e1c057) [Publication](https://www.cell.com/cell-metabolism/fulltext/S1550-4131(24)00484-4?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.1.1743558253016&__hsfp=1300058700&submissionGuid=8e58a180-37fa-4d55-a17c-1d7954a03932) Singulator 100 Singulator 200
### Maternal circadian rhythms during pregnancy dictate metabolic plasticity in offspring
[View Publication](https://www.cell.com/cell-metabolism/fulltext/S1550-4131(24)00484-4?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.1.1743558253016&__hsfp=1300058700&submissionGuid=8e58a180-37fa-4d55-a17c-1d7954a03932) [Publication](https://www.biorxiv.org/content/10.1101/2024.12.31.630834v1.full?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.2.1743558253016&__hsfp=1300058700&submissionGuid=a17e7378-367f-4fd1-9baa-242deedc0beb) Singulator 100 Singulator 200
### Chronic degenerative failure of salivary glands can be reversed through restoring mitochondrial function
[View Publication](https://www.biorxiv.org/content/10.1101/2024.12.31.630834v1.full?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.2.1743558253016&__hsfp=1300058700&submissionGuid=a17e7378-367f-4fd1-9baa-242deedc0beb) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12276987/) Singulator 200+
### Transcript-specific enrichment enables profiling rare cell states via scRNA-seq
[View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12276987/) [Publication](https://www.nature.com/articles/s41598-024-82736-6?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.4.1743558253016&__hsfp=1300058700&submissionGuid=30d7cb64-704f-4415-aa48-4016d429e620#Sec9) Singulator 100 Singulator 200
### High-throughput gene expression analysis with TempO-LINC sensitively resolves complex brain, lung and kidney heterogeneity at single-cell resolution
[View Publication](https://www.nature.com/articles/s41598-024-82736-6?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.4.1743558253016&__hsfp=1300058700&submissionGuid=30d7cb64-704f-4415-aa48-4016d429e620#Sec9) [Publication](https://www.biorxiv.org/content/10.1101/2024.12.01.626270v1.full?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.5.1743558253016&__hsfp=1300058700&submissionGuid=4cb56fc6-168a-4dd5-a3f8-8afaf354c848) Singulator 100 Singulator 200
### Multi-omic profiling identifies malignant subpopulations and a hypoxia-driven angiogenic axis as therapeutic vulnerabilities in sinonasal squamous cell carcinoma
[View Publication](https://www.biorxiv.org/content/10.1101/2024.12.01.626270v1.full?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.5.1743558253016&__hsfp=1300058700&submissionGuid=4cb56fc6-168a-4dd5-a3f8-8afaf354c848) [Publication](https://www.researchsquare.com/article/rs-5454144/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.6.1743558253016&__hsfp=1300058700&submissionGuid=cd9523ee-14ab-4574-8764-46924d882604) Singulator 100 Singulator 200
### Identification of specific microbiota members that induce beige fat biogenesis in response to dietary cues
[View Publication](https://www.researchsquare.com/article/rs-5454144/v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.6.1743558253016&__hsfp=1300058700&submissionGuid=cd9523ee-14ab-4574-8764-46924d882604) [Publication](https://www.cell.com/developmental-cell/abstract/S1534-5807(24)00661-0?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1534580724006610%3Fshowall%3Dtrue) Singulator 100 Singulator 200
### Frizzled5 controls murine intestinal epithelial cell plasticity through organization of chromatin accessibility
[View Publication](https://www.cell.com/developmental-cell/abstract/S1534-5807(24)00661-0?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1534580724006610%3Fshowall%3Dtrue) [Publication](https://aacrjournals.org/cancerres/article/85/2/231/750993/FOXR2-Targets-LHX6-DLX-Neural-Lineages-to-Drive) Singulator 100 Singulator 200
### FOXR2 targets LHX6+/DLX+ neural lineages to drive CNS neuroblastoma
[View Publication](https://aacrjournals.org/cancerres/article/85/2/231/750993/FOXR2-Targets-LHX6-DLX-Neural-Lineages-to-Drive) [Publication](https://www.nature.com/articles/s43018-024-00838-6?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.9.1743558253016&__hsfp=1300058700&submissionGuid=b7f7a640-05da-4d4a-bac8-9f4b8daf751a) Singulator 100 Singulator 200
### The neuroendocrine transition in prostate cancer is dynamic and dependent on ASCL1
[View Publication](https://www.nature.com/articles/s43018-024-00838-6?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.9.1743558253016&__hsfp=1300058700&submissionGuid=b7f7a640-05da-4d4a-bac8-9f4b8daf751a) [Publication](https://www.biorxiv.org/content/10.1101/2024.09.19.613680v2?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.10.1743558253016&__hsfp=1300058700&submissionGuid=fefae69e-e5ef-46cc-94e1-7f2a6872a7d6) Singulator 100 Singulator 200
### High-risk neuropsychiatric copy number variants are associated with convergent transcriptomic changes in human brain cells
[View Publication](https://www.biorxiv.org/content/10.1101/2024.09.19.613680v2?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.10.1743558253016&__hsfp=1300058700&submissionGuid=fefae69e-e5ef-46cc-94e1-7f2a6872a7d6) [Publication](https://www.cell.com/iscience/fulltext/S2589-0042(24)01885-6?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.11.1743558253016&__hsfp=1300058700&_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004224018856%3Fshowall%3Dtrue&submissionGuid=5f59d348-29e9-4a93-b2fe-e4b9982c32a7) Singulator 100 Singulator 200
### Atrial fibrillation variant-to-gene prioritization through cross-ancestry eQTL and single-nucleus multiomic analyses
[View Publication](https://www.cell.com/iscience/fulltext/S2589-0042(24)01885-6?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.11.1743558253016&__hsfp=1300058700&_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004224018856%3Fshowall%3Dtrue&submissionGuid=5f59d348-29e9-4a93-b2fe-e4b9982c32a7) [Publication](https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1444100/full?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.12.1743558253016&__hsfp=1300058700&submissionGuid=d76f90fe-216c-4aac-b313-7231a43c8a13) Singulator 100 Singulator 200
### Chitinase 1: a novel therapeutic target in metabolic dysfunction-associated steatohepatitis
[View Publication](https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1444100/full?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.12.1743558253016&__hsfp=1300058700&submissionGuid=d76f90fe-216c-4aac-b313-7231a43c8a13) [Publication](https://www.biorxiv.org/content/10.1101/2024.08.05.606414v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.13.1743558253016&__hsfp=1300058700&submissionGuid=4e193d20-31b9-412c-b6f3-b7bd9b02f3d5) Singulator 100 Singulator 200
### A single-nucleus census of immune and non-immune cell types for the major immune organ systems of chicken
[View Publication](https://www.biorxiv.org/content/10.1101/2024.08.05.606414v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.13.1743558253016&__hsfp=1300058700&submissionGuid=4e193d20-31b9-412c-b6f3-b7bd9b02f3d5) [Publication](https://www.biorxiv.org/content/10.1101/2024.08.03.605165v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.14.1743558253016&__hsfp=1300058700&submissionGuid=6f666125-4b9b-40ae-a7f3-013cd588958f) Singulator 100 Singulator 200
### Genomic evolution of pancreatic cancer at single-cell resolution
[View Publication](https://www.biorxiv.org/content/10.1101/2024.08.03.605165v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.14.1743558253016&__hsfp=1300058700&submissionGuid=6f666125-4b9b-40ae-a7f3-013cd588958f) [Publication](https://www.nature.com/articles/s41467-024-50660-y?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.15.1743558253016&__hsfp=1300058700&submissionGuid=a9fd91da-313a-4951-8e1d-95d0cf38654f) Singulator 100 Singulator 200
### A male mouse model for metabolic dysfunction-associated steatotic liver disease and hepatocellular carcinoma
[View Publication](https://www.nature.com/articles/s41467-024-50660-y?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.15.1743558253016&__hsfp=1300058700&submissionGuid=a9fd91da-313a-4951-8e1d-95d0cf38654f) [Publication](https://www.cell.com/iscience/fulltext/S2589-0042(24)01875-3?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.16.1743558253016&__hsfp=1300058700&_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004224018753%3Fshowall%3Dtrue&submissionGuid=5f182724-82b6-411b-bfa0-2bf14f4c64f3) Singulator 100 Singulator 200
### Single-cell landscape of long and short glandular trichomes in Nicotiana tabacum leaves
[View Publication](https://www.cell.com/iscience/fulltext/S2589-0042(24)01875-3?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.16.1743558253016&__hsfp=1300058700&_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004224018753%3Fshowall%3Dtrue&submissionGuid=5f182724-82b6-411b-bfa0-2bf14f4c64f3) [Publication](https://www.ahajournals.org/doi/10.1161/CIRCULATIONAHA.123.064719?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.17.1743558253016&__hsfp=1300058700&submissionGuid=b4207542-bea7-4f83-82ee-adae95b7ce4b) Singulator 100 Singulator 200
### Vaccine Therapy for Heart Failure Targeting the Inflammatory Cytokine Igfbp7
[View Publication](https://www.ahajournals.org/doi/10.1161/CIRCULATIONAHA.123.064719?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.17.1743558253016&__hsfp=1300058700&submissionGuid=b4207542-bea7-4f83-82ee-adae95b7ce4b) [Publication](https://bsd.biomedcentral.com/articles/10.1186/s13293-024-00628-w?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.18.1743558253016&__hsfp=1300058700&submissionGuid=ab722450-c4b5-4fb8-9824-6d715a4fe27d) Singulator 100 Singulator 200
### Sex-specific role of high-fat diet and stress on behavior, energy metabolism, and the ventromedial hypothalamus
[View Publication](https://bsd.biomedcentral.com/articles/10.1186/s13293-024-00628-w?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.18.1743558253016&__hsfp=1300058700&submissionGuid=ab722450-c4b5-4fb8-9824-6d715a4fe27d) [Publication](https://www.biorxiv.org/content/10.1101/2024.07.03.601895v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.19.1743558253016&__hsfp=1300058700&submissionGuid=9c9dd609-839a-4835-bdfe-9a55a15ddfab) Singulator 100 Singulator 200
### An orbitocortical-thalamic circuit suppresses binge alcohol-drinking
[View Publication](https://www.biorxiv.org/content/10.1101/2024.07.03.601895v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.19.1743558253016&__hsfp=1300058700&submissionGuid=9c9dd609-839a-4835-bdfe-9a55a15ddfab) [Publication](https://www.biorxiv.org/content/10.1101/2024.06.27.601103v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.20.1743558253016&__hsfp=1300058700&submissionGuid=dfdb8bf0-7f4d-4b64-909b-c4678cd4b48c) Singulator 100 Singulator 200
### Integrative Transcriptomics Reveals Layer 1 Astrocytes Altered in Schizophrenia
[View Publication](https://www.biorxiv.org/content/10.1101/2024.06.27.601103v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.20.1743558253016&__hsfp=1300058700&submissionGuid=dfdb8bf0-7f4d-4b64-909b-c4678cd4b48c) [Publication](https://www.biorxiv.org/content/10.1101/2024.05.22.595241v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.21.1743558253016&__hsfp=1300058700&submissionGuid=aa6eed6f-a4ec-4d76-bc84-dd7e2fc0fe73) Singulator 100 Singulator 200
### Genotype-to-phenotype mapping of somatic clonal mosaicism via single-cell co-capture of DNA mutations and mRNA transcripts
[View Publication](https://www.biorxiv.org/content/10.1101/2024.05.22.595241v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.21.1743558253016&__hsfp=1300058700&submissionGuid=aa6eed6f-a4ec-4d76-bc84-dd7e2fc0fe73) [Publication](https://www.biorxiv.org/content/10.1101/2024.07.01.601334v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.22.1743558253016&__hsfp=1300058700&submissionGuid=3a510c1b-ea13-4041-84cf-21b9345137b2) Singulator 100 Singulator 200
### Multiplatform molecular profiling and functional genomic screens identify prognostic signatures and mechanisms underlying MEK inhibitor response in somatic NF1 mutant glioblastoma
[View Publication](https://www.biorxiv.org/content/10.1101/2024.07.01.601334v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.22.1743558253016&__hsfp=1300058700&submissionGuid=3a510c1b-ea13-4041-84cf-21b9345137b2) [Publication](https://www.biorxiv.org/content/10.1101/2024.04.26.587806v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.23.1743558253016&__hsfp=1300058700&ct=&submissionGuid=2ff5c384-7040-4df8-91c6-30eaae489b4f) Singulator 100 Singulator 200
### High-resolution detection of copy number alterations in single cells with HiScanner
[View Publication](https://www.biorxiv.org/content/10.1101/2024.04.26.587806v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.23.1743558253016&__hsfp=1300058700&ct=&submissionGuid=2ff5c384-7040-4df8-91c6-30eaae489b4f) [Publication](https://www.nature.com/articles/s41587-024-02193-4?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.24.1743558253016&__hsfp=1300058700&submissionGuid=d4eb5dac-02d7-45d8-953c-6c79fc38c05f) Singulator 200+
### The covariance environment defines cellular niches for spatial inference
[View Publication](https://www.nature.com/articles/s41587-024-02193-4?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.24.1743558253016&__hsfp=1300058700&submissionGuid=d4eb5dac-02d7-45d8-953c-6c79fc38c05f) [Publication](https://www.science.org/doi/10.1126/sciadv.adn4649?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.25.1743558253016&__hsfp=1300058700&submissionGuid=7cb6bf68-0d64-411b-96de-7235ff6475e4) Singulator 100 Singulator 200
### Childhood cancer mutagenesis caused by transposase-derived PGBD5
[View Publication](https://www.science.org/doi/10.1126/sciadv.adn4649?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.25.1743558253016&__hsfp=1300058700&submissionGuid=7cb6bf68-0d64-411b-96de-7235ff6475e4) [Publication](https://www.biorxiv.org/content/10.1101/2023.08.19.553427v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.29.1743558253016&__hsfp=1300058700&ct=&submissionGuid=a4f220b8-1b13-4953-9b56-b675ed8faf9a) Singulator 100 Singulator 200
### Neuronal SNCA transcription during Lewy body formation
[View Publication](https://www.biorxiv.org/content/10.1101/2023.08.19.553427v1?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.29.1743558253016&__hsfp=1300058700&ct=&submissionGuid=a4f220b8-1b13-4953-9b56-b675ed8faf9a) [Publication](https://www.sciencedirect.com/science/article/pii/S0092867423001666?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.32.1743558253016&__hsfp=1300058700&submissionGuid=14a4fa98-f109-4563-98aa-4aa9ea18fe6b) Singulator 100 Singulator 200
### Single substitution in H3.3G34 alters DNMT3A recruitment to cause progressive neurodegeneration
[View Publication](https://www.sciencedirect.com/science/article/pii/S0092867423001666?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.32.1743558253016&__hsfp=1300058700&submissionGuid=14a4fa98-f109-4563-98aa-4aa9ea18fe6b) [Publication](https://www.aging-us.com/article/204435?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.34.1743558253016&__hsfp=1300058700&submissionGuid=c677f79d-e8f0-4d9f-b7f5-3458555fa9db) Singulator 100 Singulator 200
### Single nuclei profiling identifies cell specific markers of skeletal muscle aging, frailty, and senescence
[View Publication](https://www.aging-us.com/article/204435?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.34.1743558253016&__hsfp=1300058700&submissionGuid=c677f79d-e8f0-4d9f-b7f5-3458555fa9db) [Publication](https://www.nature.com/articles/s41588-022-01205-w?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.36.1743558253016&__hsfp=1300058700&submissionGuid=16d6bd6f-42f1-45ab-b755-85d2fc136d9a) Singulator 100 Singulator 200
### K27M in canonical and noncanonical H3 variants occurs in distinct oligodendroglial cell lineages in brain midline gliomas
[View Publication](https://www.nature.com/articles/s41588-022-01205-w?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.36.1743558253016&__hsfp=1300058700&submissionGuid=16d6bd6f-42f1-45ab-b755-85d2fc136d9a) [Publication](https://www.nature.com/articles/s41588-022-01088-x?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.37.1743558253016&__hsfp=1300058700&submissionGuid=a2d222b9-a609-4fd0-a304-be6a34ec2362) Singulator 100 Singulator 200
### Single-cell analyses reveal a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer
[View Publication](https://www.nature.com/articles/s41588-022-01088-x?__hstc=225964101.fe4380f775ce29a6a07134828a8c07b8.1742318073258.1743189924157.1743558253016.10&__hssc=225964101.37.1743558253016&__hsfp=1300058700&submissionGuid=a2d222b9-a609-4fd0-a304-be6a34ec2362)
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